Department of Intensive Medicine, The People's Hospital of Yuzhou City, Yuzhou, China.
Department of Kidney Transplantation, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Pharmacology. 2018;102(3-4):196-205. doi: 10.1159/000487955. Epub 2018 Aug 10.
Icariin (ICA) is a bioactive flavonoid with renal protective actions. This study investigated the effects of ICA on renal injury, inflammation, oxidative damage, apoptosis, and survival in a mouse model of cecal ligation and perforation (CLP)-induced sepsis.
Sepsis was induced by CLP. Mice were treated with ICA (30 or 60 mg/kg) for 3 days before CLP. Renal functions, inflammatory responses, oxidative damage, histological changes, apoptosis, and vascular permeability were examined. The effects of ICA on CLP-induced expression of renal nuclear factor-κB (NF-κB), cleaved caspase-3, Bax, and Bcl-2 were evaluated.
Mice in the CLP group had a low survival rate and increases in blood urea nitrogen and creatinine levels, proinflammatory cytokine levels, oxidative damage, apoptosis, and vascular permeability. These renal changes were dramatically improved by ICA treatment, especially in the 60 mg/kg ICA group. The detection of molecules involved in the inflammation and apoptosis of the kidney indicated that ICA reduced expression of NF-κB, cleaved caspase-3, and Bax but enhanced expression of Bcl-2.
ICA improves CLP-induced mortality and acute kidney injury by inhibiting renal oxidant damage, inflammatory responses, apoptosis, and vascular permeability.
淫羊藿苷（ICA）是一种具有肾脏保护作用的生物活性黄酮类化合物。本研究旨在探讨 ICA 对盲肠结扎穿孔（CLP）诱导脓毒症小鼠肾脏损伤、炎症、氧化损伤、细胞凋亡和存活的影响。
CLP 诱导脓毒症。CLP 前 3 天用 ICA（30 或 60mg/kg）处理小鼠。检测肾功能、炎症反应、氧化损伤、组织学变化、细胞凋亡和血管通透性。评估 ICA 对 CLP 诱导的肾核因子-κB（NF-κB）、裂解 caspase-3、Bax 和 Bcl-2 表达的影响。
CLP 组小鼠存活率低，血尿素氮和肌酐水平、促炎细胞因子水平、氧化损伤、细胞凋亡和血管通透性升高。ICA 治疗显著改善了这些肾脏变化，尤其是在 60mg/kg ICA 组。检测肾脏炎症和凋亡相关分子表明，ICA 降低了 NF-κB、裂解 caspase-3 和 Bax 的表达，而增强了 Bcl-2 的表达。
ICA 通过抑制肾脏氧化损伤、炎症反应、细胞凋亡和血管通透性，改善 CLP 诱导的死亡率和急性肾损伤。
