Honoré T, Drejer J, Nielsen M
Neurosci Lett. 1986 Mar 28;65(1):47-52. doi: 10.1016/0304-3940(86)90118-7.
Binding of [3H]kainate to the kainate subtype of excitatory amino acid receptors revealed two binding sites. The high-affinity site (Kd = 3.5 nM) was sensitive to calcium ions and has a molecular target size as determined by high-energy radiation inactivation analysis of 76,600 daltons. The low-affinity site (Kd = 65 nM) was insensitive to calcium ions and has a molecular target size similar to the quisqualate-subtype of excitatory amino acid receptors. The change in binding parameters of the two sites with radiation dose strongly suggests that low affinity, calcium-insensitive [3H]kainate binding sites are equivalent to quisqualate sites.
[3H]海人酸与兴奋性氨基酸受体的海人酸亚型结合显示出两个结合位点。高亲和力位点（Kd = 3.5 nM）对钙离子敏感，通过高能辐射失活分析确定其分子靶标大小为76,600道尔顿。低亲和力位点（Kd = 65 nM）对钙离子不敏感，其分子靶标大小与兴奋性氨基酸受体的quisqualate亚型相似。两个位点的结合参数随辐射剂量的变化强烈表明，低亲和力、对钙不敏感的[3H]海人酸结合位点等同于quisqualate位点。
