Thalmann I, Thallinger G, Comegys T H, Thalmann R
ORL J Otorhinolaryngol Relat Spec. 1986;48(2):107-15. doi: 10.1159/000275855.
Evidence is presented that, in contrast to the traditional view, a large proportion of the proteins of the tectorial membrane (TM) consists of collagen, primarily type II: characteristic amino acid composition of TM, including high levels of glycine, hydroxyproline and hydroxylysine; comigration of the main TM protein with appropriate collagen standards in two-dimensional gel electrophoresis; digestion of the main TM protein band following treatment with bacterial collagenase; one- and two-dimensional peptide mapping of cyanogen bromide digests of the TM exhibits patterns characteristic for collagen type II.
有证据表明,与传统观点相反,盖膜(TM)的大部分蛋白质由胶原蛋白组成,主要是II型胶原蛋白:TM的特征性氨基酸组成,包括高水平的甘氨酸、羟脯氨酸和羟赖氨酸;在二维凝胶电泳中,主要的TM蛋白与合适的胶原蛋白标准品共迁移;用细菌胶原酶处理后,主要的TM蛋白条带被消化;TM的溴化氰消化产物的一维和二维肽图显示出II型胶原蛋白的特征模式。