Rice Research Institute, Key Laboratory of Application and Safety Control of Genetically Modified Crops, Academy of Agricultural Sciences, Southwest University, Chongqing, China.
J Exp Bot. 2018 Sep 14;69(20):4791-4804. doi: 10.1093/jxb/ery250.
The de novo synthesis of purine nucleotides is crucial to all living organisms, but limited information is available for plants. In this study, we isolated a virescent-albino leaf 1 (val1) mutant of rice (Oryza sativa) that produces dynamic green-revertible albino and narrow-leaf phenotypes. In albino leaves, chloroplast development was defective, pigment contents were reduced, and cell division was impaired compared with the wild-type. Map-based cloning revealed that VAL1 encodes a phosphoribosylamine-glycine ligase (PurD), the second enzyme in the de novo purine biosynthesis pathway. Subcellular localization analysis demonstrated that VAL1 was localized in the chloroplast. Our results demonstrate that VAL1 is a crucial enzyme in the de novo purine biosynthesis pathway and is involved in regulating chloroplast development, chlorophyll metabolism, and cell division during leaf development in rice.
从头合成嘌呤核苷酸对所有生物都至关重要，但有关植物的信息有限。在这项研究中，我们分离了一个水稻（Oryza sativa）的黄绿叶 1（val1）突变体，该突变体产生动态的绿色可逆白化和窄叶表型。在白化叶片中，与野生型相比，叶绿体发育缺陷、色素含量降低且细胞分裂受损。基于图谱的克隆表明，VAL1 编码一个磷酸核糖胺-甘氨酸连接酶（PurD），这是从头嘌呤生物合成途径中的第二个酶。亚细胞定位分析表明，VAL1 定位于叶绿体。我们的结果表明，VAL1 是从头嘌呤生物合成途径中的关键酶，参与调控水稻叶片发育过程中的叶绿体发育、叶绿素代谢和细胞分裂。
