Wei Yu, Li Xiaoqiong, Li Dongxiu, Su Xuejun, Huang Yunchuan, Li Qiuwen, Liang Manling, Yang Xinghai
Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
State Key Laboratory for Conservation and Utillzation of Subtropical Agro-Bioresources, Nanning 530007, China.
Curr Issues Mol Biol. 2024 Jun 27;46(7):6508-6521. doi: 10.3390/cimb46070388.
Chloroplasts are organelles responsible for photosynthesis in plants, providing energy for growth and development. However, the genetic regulatory mechanisms underlying early chloroplast development in rice remain incompletely understood. In this study, we identified a rice seedling thermosensitive chlorophyll-deficient mutant, , and the genetic analysis of two F populations suggested that this trait may be controlled by more than one pair of alleles. Through reciprocal F populations and QTL-seq technology, was mapped to the interval of 24,280,402-25,920,942 bp on rice chromosome 8, representing a novel albino gene in rice. Within the candidate gene region of , there were 258 predicted genes, among which , , and encode pentatricopeptide repeat (PPR) proteins. RNA-seq identified 18 DEGs (differentially expressed genes) within the candidate interval, with showing homology to the pigment biosynthesis-related genes and ; and were implicated in chlorophyll precursor synthesis. RT-qPCR was employed to assess the expression levels of , , , , , and in the wild-type and mutant plants. Among them, the differences in the expression levels of and were the most significant. This study will contribute to further elucidating the molecular mechanisms of rice chloroplast development.
叶绿体是植物进行光合作用的细胞器，为植物的生长和发育提供能量。然而，水稻早期叶绿体发育的遗传调控机制仍未完全明确。在本研究中，我们鉴定出一个水稻幼苗温敏叶绿素缺乏突变体，两个F群体的遗传分析表明该性状可能受多对等位基因控制。通过正反交F群体和QTL-seq技术，将该突变体定位到水稻第8号染色体上24,280,402 - 25,920,942 bp区间，这代表了水稻中一个新的白化基因。在该突变体的候选基因区域内，有258个预测基因，其中，、和编码五肽重复（PPR）蛋白。RNA测序在候选区间内鉴定出18个差异表达基因（DEGs），其中与色素生物合成相关基因和具有同源性；和参与叶绿素前体合成。采用RT-qPCR检测了野生型和突变体植株中、、、、和的表达水平。其中，和的表达水平差异最为显著。本研究将有助于进一步阐明水稻叶绿体发育的分子机制。
