INSERM U1043 - CNRS UMR 5282, CPTP.
Laboratoire de Virologie, CHU Toulouse Purpan, Toulouse.
AIDS. 2018 Oct 23;32(16):2429-2431. doi: 10.1097/QAD.0000000000001976.
: Next-generation sequencing is a sensitive method for determining HIV-1 tropism but there is a lack of data on the quantification of X4 variants. We evaluated MiSeq and 454 GS-Junior platforms for determining HIV-1 tropism and for quantifying X4 variants. Both platforms were 93% concordant for determining HIV-1 tropism and correlated well for determining the proportion of X4 variants (Spearman correlation, ρ = 0.748; P < 0.0001). MiSeq Illumina sequencing seems to be well adapted for characterizing X4-containing samples.
下一代测序是一种用于确定 HIV-1 嗜性的敏感方法，但缺乏关于 X4 变体定量的数据分析。我们评估了 MiSeq 和 454 GS-Junior 平台来确定 HIV-1 嗜性和定量 X4 变体。两种平台在确定 HIV-1 嗜性方面的一致性为 93%，在确定 X4 变体比例方面相关性良好（Spearman 相关系数，ρ=0.748；P<0.0001）。MiSeq Illumina 测序似乎非常适合用于描述包含 X4 的样本。
