Giambrone J J, Clay R P
Poult Sci. 1986 Mar;65(3):457-61. doi: 10.3382/ps.0650457.
The efficacy of various reovirus vaccination programs in commercial broiler breeder pullets for preventing clinical reovirus infections in broiler progeny was studied in three experiments. In each, 70 1-day-old broilers from each of four different commercial poultry companies that used various combinations of live and killed reovirus vaccines in breeder pullets were used. Broiler progeny from each company were bled and serum analyzed for neutralization antibody to the S1133 reovirus. Twenty chicks from each company were challenged by foot pad with a virulent reovirus (S1133) and 20 chicks challenged with another reovirus (81-B) isolate to determine their susceptibility. An additional 20 chicks from each of the four groups were maintained as unchallenged controls. Chicks were examined for morbidity, mortality, and weight gain for a 3-week period. Three weeks after challenge, all chickens were necropsied for gross lesions. There was a direct correlation between the number of doses of live inactivated reovirus vaccines that pullets received to antibody titer and resistance to challenge in the day-old progeny. Day-old progeny derived from breeder pullets that received one dose of live and two doses of inactivated reovirus vaccines had the highest numerical maternal antibody titer and best resistance to clinical infection following challenge.
在三项实验中研究了各种呼肠孤病毒疫苗接种方案对商品肉种鸡雏鸡预防肉仔鸡后代临床呼肠孤病毒感染的效果。每项实验中，使用了来自四家不同商业家禽公司的70只1日龄肉仔鸡，这些公司在种鸡雏鸡中使用了活疫苗和灭活呼肠孤病毒疫苗的各种组合。对每家公司的肉仔鸡后代进行采血，并分析血清中针对S1133呼肠孤病毒的中和抗体。每家公司的20只雏鸡通过脚垫接种强毒呼肠孤病毒（S1133），20只雏鸡接种另一种呼肠孤病毒（81 - B）分离株，以确定它们的易感性。来自四组中的每组另外20只雏鸡作为未接种对照饲养。对雏鸡进行为期3周的发病率、死亡率和体重增加情况检查。接种后3周，对所有鸡进行剖检以检查大体病变。种鸡雏鸡接种的活灭活呼肠孤病毒疫苗剂量数量与1日龄后代的抗体滴度和对攻毒的抵抗力之间存在直接相关性。来自接受一剂活疫苗和两剂灭活呼肠孤病毒疫苗的种鸡雏鸡的1日龄后代具有最高的母源抗体滴度数值，并且在攻毒后对临床感染具有最佳抵抗力。
