ITHPP-Alcen, Hameau de Drèle, Thégra, France.
Institut de Pharmacologie et Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, Toulouse, France.
PLoS One. 2018 Aug 13;13(8):e0201448. doi: 10.1371/journal.pone.0201448. eCollection 2018.
Bacterial spores are one of the most resilient life forms on earth and are involved in many human diseases, such as infectious diarrhea, fatal paralytic illnesses and respiratory infections. Here, we investigated the mechanisms involved in the death of Bacillus pumilus spores after exposure to electric arcs in water. Cutting-edge microscopies at the nanoscale did not reveal any structural disorganization of spores exposed to electric arcs. This result suggested the absence of physical destruction by a propagating shock wave or an exposure to an electric field. However, Pulsed-Field Gel Electrophoresis (PFGE) revealed genomic DNA damage induced by UV radiation and Reactive Oxygen Species (ROS). UV induced single-strand DNA breaks and thymine dimers while ROS were mainly involved in base excision. Our findings revealed a correlation between DNA damage and the treatment of spores with electrical discharges.
细菌孢子是地球上最具弹性的生命形式之一，与许多人类疾病有关，如感染性腹泻、致命性麻痹性疾病和呼吸道感染。在这里，我们研究了在水中电弧作用下导致短小芽孢杆菌孢子死亡的机制。在纳米尺度的尖端显微镜下，没有发现暴露在电弧下的孢子结构紊乱。这一结果表明，传播的冲击波或电场没有造成物理破坏。然而，脉冲场凝胶电泳（PFGE）显示了由紫外线辐射和活性氧（ROS）引起的基因组 DNA 损伤。紫外线诱导单链 DNA 断裂和胸腺嘧啶二聚体，而 ROS 主要参与碱基切除。我们的研究结果揭示了 DNA 损伤与用放电处理孢子之间的相关性。
