Department of Functional and Structural Biology, Institute of Biology, University of Campinas - UNICAMP, Campinas, SP, Brazil.
Department of Chemistry and Environmental Sciences, Institute of Biosciences, Humanities and Exact Sciences, São Paulo State University - UNESP, São José do Rio Preto, SP, Brazil.
Reprod Toxicol. 2018 Oct;81:168-179. doi: 10.1016/j.reprotox.2018.08.011. Epub 2018 Aug 11.
This study evaluated the consequences of gestational exposure to di-n-butyl phthalate (DBP) for testicular steroidogenesis and sperm parameters of the adult gerbil and the interference of corn oil (co), a vehicle widely used for administration of liposoluble agents, on DBP effects. Pregnant gerbils received no treatment or were treated from gestational day 8 to 23 via gavage with 0.1 mL/day of co only or containing DBP (100 mg/kg/day). Maternal co intake enhanced serum estradiol levels and testicular content of ERα, and reduced sperm reserve of adult offspring. Gestational DBP exposure caused dyslipidemia, increased serum and intratesticular estradiol levels and reduced sperm reserve and motility. Thus, maternal co supplementation alters circulating estradiol and impairs sperm quantity and quality of offspring. Gestational DBP exposure alters lipid metabolism and testicular steroidogenesis and worsens the negative effects of co on the sperm reserve and motility of gerbil. Therefore, co interferes with the reproductive response to DBP.
本研究评估了妊娠暴露于邻苯二甲酸二丁酯（DBP）对成年沙鼠睾丸类固醇生成和精子参数的影响，以及作为脂溶性药物常用给药载体的玉米油（CO）对 DBP 作用的干扰。妊娠沙鼠通过灌胃接受以下处理：从妊娠第 8 天至 23 天每天接受 0.1 mL 的 CO 处理或含有 DBP（100mg/kg/天）的 CO 处理。母体 CO 摄入增加了血清雌二醇水平和睾丸 ERα 含量，并减少了成年后代的精子储备。妊娠 DBP 暴露导致血脂异常，增加了血清和睾丸内的雌二醇水平，并降低了精子储备和活力。因此，母体 CO 补充改变了循环中的雌二醇，并损害了后代的精子数量和质量。妊娠 DBP 暴露改变了脂质代谢和睾丸类固醇生成，并加重了 CO 对沙鼠精子储备和活力的负面影响。因此，CO 干扰了对 DBP 的生殖反应。
