Xi Hui-Rong, Yang Fang-Fang, Gao Yu-Hai, Ma Hui-Ping, Chen Ke-Ming
Institute of Orthopaedics, Lanzhou General Hospital of PLA, Lanzhou 730050, Gansu, China;
Zhongguo Gu Shang. 2018 Jul 25;31(7):635-641. doi: 10.3969/j.issn.1003-0034.2018.07.010.
To compare effects of resveratrol, puerarin and the compounds on peak bone mass in rats.
Forty SPF Wistar rats weighed 109.45 g to 119.44 g with an average of 115.87 g were selected. After 3 days' adaption, rats were divided into control group (the same volume of distilled water per day), puerarin group(15.4 mg/kg puerarin daily), resveratrol group (8.4 mg/kg resveratrol daily), compound drug group (daily dose of 8.4 resveratrol added 15.4 mg/kg of puerarin) and 10 in each group. The body weight of the rats was monitored at every 7 days and body bone density was measured at every month. All rats were sacrificed after 3 months. The bone mineral density of femur and vertebrae was detected by dual energy X-ray absorptiometry; bone biomechanics, VG staining was used to analyze bone histomorphometry;ELISA was used to detect serum bone metabolic index and microstructure of femur were scanned with Micro-CT scanner.
There were no significant differences in body weight among groups during exoeriment. Bone mineral density results showed BMD of femur and vertebrae in the other three groups were significantly increased, and R+P group was significantly higher than PR group and RES group(<0.05) by compared with CON group;three-point bending and compression test results showed compared with CON group, other three groups of femoral and vertebral maximum load values were significantly increased, and P+R group was higher than PR group and RES group, but elastic modulus was not statistically significant. Bone histomorphometry showed that number of trabecular bone in other three groups were significantly increased compared with CON group, separation of trabecular bone were significantly reduced, continuity was improved, and R+P group was significantly better than RES and PR group. The results of Micro-CT scan showed that separation of trabecular bone were significantly reduced, continuity were improved in other three groups, and R+P group was significantly better than RES and PR group. The numbers of trabecular bone (Tb.N), trabecular bone thickness (Tb.Th), volume of trabecular bone (BV/TV) in PR group, RES group and R+P group were significantly higher than CON group, but trabecular bone separation (Tb.Sp) was significantly reduced. Serum levels results showed, level of OC in the other three groups were higher than control group(<0.05), content of TRACP 5b decreased, and level of OC in P+R group was significantly higher than PR group and RES group, content of TRACP 5b was no significant change.
Compound of puerarin and resveratrol assigned in a 1:1 ratio could improve bone mineral density and bone mass in young rats, enhance biomechanical properties of bone, promote mineralization and maturation of osteoblasts, inhibit osteoblastic bone resorption, and is better than the role of their respective monomers. The paper showed that traditional Chinese medicine compound medicine will be used as a new way to prevent and treat osteoporosis.
比较白藜芦醇、葛根素及其复方对大鼠峰值骨量的影响。
选取40只SPF级Wistar大鼠，体重109.45 g至119.44 g，平均体重115.87 g。适应性喂养3天后，将大鼠分为对照组（每日给予等体积蒸馏水）、葛根素组（每日给予15.4 mg/kg葛根素）、白藜芦醇组（每日给予8.4 mg/kg白藜芦醇）、复方药物组（每日剂量为8.4 mg白藜芦醇加15.4 mg/kg葛根素），每组10只。每7天监测大鼠体重，每月测量大鼠体骨密度。3个月后处死所有大鼠。采用双能X线吸收法检测股骨和椎骨的骨密度；采用骨生物力学、VG染色分析骨组织形态计量学；采用ELISA法检测血清骨代谢指标，并用Micro-CT扫描仪扫描股骨的微观结构。
实验期间各组大鼠体重无显著差异。骨密度结果显示，与对照组相比，其他三组的股骨和椎骨骨密度均显著升高，且复方组显著高于葛根素组和白藜芦醇组（P<0.05）；三点弯曲和压缩试验结果显示，与对照组相比，其他三组的股骨和椎骨最大载荷值均显著升高，且复方组高于葛根素组和白藜芦醇组，但弹性模量无统计学差异。骨组织形态计量学显示，与对照组相比，其他三组的骨小梁数量显著增加，骨小梁间距显著减小，连续性改善，且复方组显著优于白藜芦醇组和葛根素组。Micro-CT扫描结果显示，其他三组的骨小梁间距显著减小，连续性改善，且复方组显著优于白藜芦醇组和葛根素组。复方组、葛根素组和白藜芦醇组的骨小梁数量（Tb.N）、骨小梁厚度（Tb.Th）、骨小梁体积（BV/TV）均显著高于对照组，但骨小梁间距（Tb.Sp）显著减小。血清学结果显示，其他三组的骨钙素水平均高于对照组（P<0.05），抗酒石酸酸性磷酸酶5b含量降低，且复方组的骨钙素水平显著高于葛根素组和白藜芦醇组，抗酒石酸酸性磷酸酶5b含量无显著变化。
1:1配比的葛根素与白藜芦醇复方可提高幼龄大鼠的骨密度和骨质量，增强骨生物力学性能，促进成骨细胞矿化和成熟，抑制破骨细胞骨吸收，且优于各自单体的作用。本文表明中药复方将成为防治骨质疏松的新途径。
