Cheng Kui, Ge Bao-Feng, Zhen Ping, Chen Ke-Ming, Ma Xiao-Ni, Zhou Jian, Song Peng, Ma Hui-Ping
Zhongguo Gu Shang. 2014 Jul;27(7):587-91.
To compare the ability of osthole (OST) and genistein (GEN) in enhancing bone peak bone mass of rats to prevent osteoporosis.
Thirty-six female one-month-old SD rats of (125 +/- 3) g body weight were randomly divided into three groups, 12 rats in each group, one group was orally administered osthole at 9 mg x kg(-1) d(-1), one group was given genistein at 10 mg x kg(-1) d(-1) and another was given equal quantity of distilled water as the control. The body weight was monitored weekly and the bone mineral density (BMD) of total body was measured every month. All rats were sacrificed after three months, the femoral bone mineral density, the serum levels of osteocalcin (OC) and anti-tartaric acid phosphatase 5b (TRACP 5b) were measured by Elisa. The bone microarchitectures were analyzed with micro-CT and the bone biomechanics properties were tested with universal material machine.
No significant differences were observed between O-treated or GEN group and the control for the food-intake and body weight during three months. However, the rats treated with OST had significant higher BMD for both total body and femur than the control and GEN group. The O-treated rats also had higher level of serum OC and lower level of TRACP 5b. Besides, they owned bigger bone volume/tissue volume, trabecular thickness, trabecular number but smaller trabecular spacing. In the three point bending tests of femurs,they were found to have larger maximum load, the young's modulus and structural model index (SMI).
Orally administered osthole could efficiently increase the peak bone mass of rats,which provide new ideas for preventing osteoporosis.
比较蛇床子素（OST）和染料木黄酮（GEN）提高大鼠骨峰值骨量以预防骨质疏松症的能力。
将36只体重为（125±3）g的1月龄雌性SD大鼠随机分为3组，每组12只。一组大鼠按9mg·kg⁻¹·d⁻¹的剂量口服蛇床子素，一组按10mg·kg⁻¹·d⁻¹的剂量给予染料木黄酮，另一组给予等量蒸馏水作为对照。每周监测体重，每月测量全身骨密度（BMD）。3个月后处死所有大鼠，采用酶联免疫吸附测定法（ELISA）检测股骨骨密度、血清骨钙素（OC）和抗酒石酸酸性磷酸酶5b（TRACP 5b）水平。用显微CT分析骨微结构，用万能材料试验机测试骨生物力学性能。
在3个月期间，OST治疗组或GEN组与对照组在食物摄入量和体重方面未观察到显著差异。然而，与对照组和GEN组相比，接受OST治疗的大鼠全身和股骨的BMD显著更高。接受OST治疗的大鼠血清OC水平也更高，TRACP 5b水平更低。此外，它们的骨体积/组织体积、骨小梁厚度、骨小梁数量更大，但骨小梁间距更小。在股骨三点弯曲试验中，发现它们具有更大的最大载荷、杨氏模量和结构模型指数（SMI）。
口服蛇床子素可有效增加大鼠的峰值骨量，为预防骨质疏松症提供新思路。
