Wang Jianguo, Zhu Xiaoyan, She Guanghui, Kong Yezi, Guo Yazhou, Wang Zhe, Liu Guowen, Zhao Baoyu
College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China.
UMR 1195 Inserm and University Paris-Saclay, Kremlin-Bicêtre, France.
BMC Vet Res. 2018 Aug 13;14(1):236. doi: 10.1186/s12917-018-1560-7.
During peripartum period, dairy cows are highly susceptible to energy metabolism disorders such as fatty liver and ketosis. Angiopoietin-like protein 4 (ANGPTL4) and fibroblast growth factor 21 (FGF21), known as hepatokines, play important roles in lipid metabolism. The purposes of our study were to evaluate variations of serum ANGPTL4 and FGF21 concentrations in periparturient dairy cows and changes in these serum analyte concentrations of energy-related metabolic disorders in early lactation dairy cows. This study was divided into two experiments. Experiment I: Blood parameters were measured in healthy periparturient Holstein cows from 4 wk antepartum to 4 wk postpartum (n = 219). In this experiment, weekly blood samples were obtained from 4 wk before the expected calving date through 4 wk after calving. Experiment II: Blood parameters were measured in healthy cows (n = 30) and cows with clinical ketosis (n = 29) and fatty liver (n = 25) within the first 4 wk of lactation. In the present study, all blood samples were collected from the coccygeal vein in the early morning before feeding.
Serum ANGPTL4 and FGF21 concentrations peaked at parturition, and declined rapidly over the following 2 wk Serum ANGPTL4 and FGF21 concentrations were positively correlated with serum non-esterified fatty acids (NEFA) concentration (r = 0.856, P = 003; r = 0.848, P = 0.004, respectively). Cows with clinical ketosis and fatty liver had significantly higher serum ANGPTL4 and FGF21 concentrations than healthy cows (P < 0.01).
Serum ANGPTL4 and FGF21 concentrations were elevated during peripartum period, suggesting that energy balance changes that were associated with parturition contributed significantly to these effects. Although FGF21 and ANGPTL4 could play important roles in the adaptation of energy metabolism, they may be involved in the pathological processes of energy metabolism disorders of dairy cows in the peripartum period.
在围产期，奶牛极易患能量代谢紊乱疾病，如脂肪肝和酮病。血管生成素样蛋白4（ANGPTL4）和成纤维细胞生长因子21（FGF21）作为肝脏因子，在脂质代谢中发挥重要作用。我们研究的目的是评估围产期奶牛血清ANGPTL4和FGF21浓度的变化，以及早期泌乳奶牛这些血清分析物浓度与能量相关代谢紊乱的变化。本研究分为两个实验。实验I：在产前4周至产后4周的健康围产期荷斯坦奶牛中测量血液参数（n = 219）。在该实验中，从预期产犊日期前4周至产后4周每周采集血液样本。实验II：在泌乳期前4周内，对健康奶牛（n = 30）、临床酮病奶牛（n = 29）和脂肪肝奶牛（n = 25）测量血液参数。在本研究中，所有血液样本均在清晨喂食前从尾静脉采集。
血清ANGPTL4和FGF21浓度在分娩时达到峰值，并在接下来的2周内迅速下降。血清ANGPTL4和FGF21浓度与血清非酯化脂肪酸（NEFA）浓度呈正相关（分别为r = 0.856，P = 0.003；r = 0.848，P = 0.004）。临床酮病和脂肪肝奶牛的血清ANGPTL4和FGF21浓度显著高于健康奶牛（P < 0.01）。
围产期血清ANGPTL4和FGF21浓度升高，表明与分娩相关的能量平衡变化对这些影响有显著贡献。尽管FGF21和ANGPTL4可能在能量代谢适应中发挥重要作用，但它们可能参与了围产期奶牛能量代谢紊乱的病理过程。
