亚致死剂量照射诱导的淋巴细胞缺乏症于……中（此处原文不完整）

Lymphocyte Deficiency Induced by Sublethal Irradiation in .

作者信息

Rollins-Smith Louise A, Robert Jacques

机构信息

Departments of Pathology, Microbiology, and Immunology and of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232;

Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee 37235.

出版信息

Cold Spring Harb Protoc. 2019 Jan 2;2019(1):pdb.prot097626. doi: 10.1101/pdb.prot097626.

DOI:10.1101/pdb.prot097626

PMID:30104411

Abstract

In many studies of diseases affecting amphibians, it is important to determine to what extent lymphocyte-mediated defenses are involved. For example, in studies of the nature of the immune response of to the amphibian chytrid fungus, , it was essential to determine if mucosal antimicrobial peptides or lymphocyte-mediated immunity was most important for resistance to this skin pathogen. In this protocol, we describe a method for sublethal irradiation to reduce lymphocyte numbers. Briefly, adults or tadpoles are exposed to 9 Gy (900 rads) of irradiation applied by exposure to a cesium source or gamma irradiator to reduce lymphocyte populations in the spleen.

摘要

在许多关于影响两栖动物疾病的研究中，确定淋巴细胞介导的防御机制在多大程度上起作用很重要。例如，在对两栖类壶菌免疫反应性质的研究中，确定黏膜抗菌肽或淋巴细胞介导的免疫对于抵抗这种皮肤病原体是否最为重要至关重要。在本方案中，我们描述了一种亚致死剂量辐照以减少淋巴细胞数量的方法。简而言之，成年两栖动物或蝌蚪暴露于通过铯源或伽马辐照器施加的9戈瑞（900拉德）辐照下，以减少脾脏中的淋巴细胞数量。