Department of Anesthesiology, Changhua Christian Hospital, Changhua 50006, Taiwan.
Transplant Medicine & Surgery Research Center, Changhua Christian Hospital, Changhua 50006, Taiwan.
Molecules. 2018 Aug 13;23(8):2016. doi: 10.3390/molecules23082016.
Ursolic acid (UA), is a kind of triterpene acid that exhibits wide biological properties. In this article, the effects of UA on apoptosis and the proliferation of human hepatoma Huh-7 cells were reported. The MTT results showed that cell viability of Huh-7 was reduced in a concentration and time-dependent effect. In addition, DAPI staining was used to detected condensation of chromatin in nucleus. Apoptotic cell population was examined using Annexin V/PI staining. The results showed that exposure to UA affected extrinsic and intrinsic pathways through, reduced expression of Bcl-2, Mcl-1, and TCTP; increased levels of the apoptotic proteins TNF-α, Fas, FADD, and Bax; and activation of cleaved caspase-3 and PARP. UA also inhibited the p-Akt and p38 MAPK signaling transduction pathways, and increased activity in the p-ERK signaling pathway. Taken together, UA inhibited the cell growth of Huh-7 cells and affected apoptosis, via regulated cellular signaling transduction.
熊果酸（UA）是一种具有广泛生物学特性的三萜酸。本文报道了 UA 对人肝癌 Huh-7 细胞凋亡和增殖的影响。MTT 结果表明，细胞活力呈浓度和时间依赖性降低。此外，还使用 DAPI 染色检测核染色质的凝聚。使用 Annexin V/PI 染色检测凋亡细胞群体。结果表明，UA 通过降低 Bcl-2、Mcl-1 和 TCTP 的表达，以及增加 TNF-α、Fas、FADD 和 Bax 等凋亡蛋白的水平，影响外源性和内源性途径，从而影响细胞凋亡。同时，UA 还抑制了 p-Akt 和 p38 MAPK 信号转导通路，并激活了 cleaved caspase-3 和 PARP。UA 还抑制了 Huh-7 细胞的细胞生长并影响凋亡，通过调节细胞信号转导。
