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榛细胞悬浮培养物来源的诱导子增强了 Neocosmospora 黑曲霉的生长和紫杉醇的生产。

Elicitors Derived from Hazel (Corylus avellana L.) Cell Suspension Culture Enhance Growth and Paclitaxel Production of Epicoccum nigrum.

机构信息

Plant Breeding and Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, P.O. Box 14115-336, Iran.

Plant Pathology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, P.O. Box 14115-336, Iran.

出版信息

Sci Rep. 2018 Aug 13;8(1):12053. doi: 10.1038/s41598-018-29762-3.

DOI:10.1038/s41598-018-29762-3
PMID:30104672
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6089963/
Abstract

The microbial fermentation is considered as the potential source for large-scale production of paclitaxel. Since co-cultivation/mixed fermentation strategy has been reported as a yield enhancement strategy for paclitaxel production, investigation of fungal endophyte response to plant culture medium, plant cell extract (CE) and medium filtrate (MF) of plant cell suspension culture in terms of growth and paclitaxel production is interesting. In this study, 35 endophytic fungi were isolated from Taxus baccata and Corylus avellana grown in Iran. The analysis of high-performance liquid chromatography and mass spectrometry showed that one isolate (YEF) produced paclitaxel. The isolate YEF was identified as Epicoccum nigrum by sequencing of ITS1-5.8S-ITS2 rDNA region and actin gene. YEF was slow-growing in Murashige and Skoog medium, but the synergistic interaction of gibberellic acid (GA) and CE of C. avellana enhanced the growth of YEF. The highest total yield of paclitaxel (314.7 µg/l; 11.5-folds) of E. nigrum strain YEF was obtained by using 28% (v/v) filter sterilized CE of C. avellana and 2 µg ml GA that was significantly higher than the control. In this study, the effects of the plant cell extract on growth and paclitaxel production of paclitaxel producing endophytic fungus were studied for the first time.

摘要

微生物发酵被认为是大规模生产紫杉醇的潜在来源。由于共培养/混合发酵策略已被报道为提高紫杉醇生产的产量增强策略，因此研究真菌内生菌对植物培养基、植物细胞提取物 (CE) 和植物细胞悬浮培养的培养基滤液 (MF) 的生长和紫杉醇生产的反应是很有趣的。在这项研究中，从伊朗生长的红豆杉和榛子中分离出 35 种内生真菌。高效液相色谱和质谱分析表明，一种分离株 (YEF) 产生了紫杉醇。通过 ITS1-5.8S-ITS2 rDNA 区和肌动蛋白基因的测序，将分离株 YEF 鉴定为黑曲霉 (Epicoccum nigrum)。YEF 在 Murashige 和 Skoog 培养基中生长缓慢，但赤霉素 (GA) 和榛子 CE 的协同作用增强了 YEF 的生长。通过使用 28%（v/v）过滤灭菌的榛子 CE 和 2 µg/ml GA，黑曲霉菌株 YEF 的紫杉醇总产量（314.7 µg/l；11.5 倍）达到最高，明显高于对照。在这项研究中，首次研究了植物细胞提取物对产紫杉醇内生真菌生长和紫杉醇生产的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fff/6089963/debd9c77b86c/41598_2018_29762_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fff/6089963/7424ce4c8602/41598_2018_29762_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fff/6089963/97cde1cd807c/41598_2018_29762_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fff/6089963/7d3d076ddea7/41598_2018_29762_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fff/6089963/a5d197d44250/41598_2018_29762_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fff/6089963/debd9c77b86c/41598_2018_29762_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fff/6089963/7424ce4c8602/41598_2018_29762_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fff/6089963/97cde1cd807c/41598_2018_29762_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fff/6089963/7d3d076ddea7/41598_2018_29762_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fff/6089963/a5d197d44250/41598_2018_29762_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fff/6089963/debd9c77b86c/41598_2018_29762_Fig5_HTML.jpg

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