Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Pasteura 5, 02-093 Warsaw, Poland.
Chem Commun (Camb). 2018 Aug 28;54(70):9773-9776. doi: 10.1039/c8cc04968h.
We describe a new type of nucleotide-derived fluorescent probe designed for monitoring pyrophosphatase activity based on excimer-to-monomer transitions, called ExciTide. The nucleotides were designed with two self-interacting dye moieties and synthesised using copper-catalysed azide-alkyne cycloaddition click chemistry. We applied these probes for enzyme activity monitoring and inhibitor evaluation. Some of the probes permeated into living cells, yielding interesting prospects for future applications.
我们描述了一种新型核苷酸衍生荧光探针，用于基于激基缔合物-单体转变监测焦磷酸酶活性，称为 ExciTide。这些核苷酸设计有两个自相互作用的染料部分，并使用铜催化的叠氮-炔环加成点击化学合成。我们将这些探针应用于酶活性监测和抑制剂评估。一些探针渗透进入活细胞，为未来的应用提供了有趣的前景。
