Opt Lett. 2018 Aug 15;43(16):4057-4060. doi: 10.1364/OL.43.004057.
Label-free particle analysis is a powerful tool in chemical, pharmaceutical, and cosmetic industries as well as in basic sciences, but its throughput is significantly lower than that of fluorescence-based counterparts. Here we present a label-free single-particle analyzer based on broadband dual-comb coherent Raman scattering spectroscopy operating at a spectroscopic scan rate of 10 kHz. As a proof-of-concept demonstration, we perform broadband coherent anti-Stokes Raman scattering measurements of polystyrene microparticles flowing on an acoustofluidic chip at a high throughput of >1000 particles per second. This high-throughput label-free particle analyzer has the potential for high-precision statistical analysis of a large number of microparticles including biological cells.
无标记粒子分析是化学、制药和化妆品行业以及基础科学领域的有力工具，但它的通量明显低于基于荧光的同类工具。在这里，我们展示了一种基于宽带双梳相干拉曼散射光谱学的无标记单粒子分析仪，其光谱扫描速率为 10 kHz。作为概念验证演示，我们在声学微流控芯片上以 >1000 个/秒的高通量对聚苯乙烯微球进行宽带相干反斯托克斯拉曼散射测量。这种高通量无标记粒子分析仪具有对大量微球（包括生物细胞）进行高精度统计分析的潜力。
