Fernandes Yohaan, Rampersad Mindy, Eberhart Johann K
University of Texas at Austin, 2401 Speedway, Patterson Hall Room 522, Austin, TX 78712, United States of America.
Behav Brain Res. 2019 Jan 1;356:46-50. doi: 10.1016/j.bbr.2018.08.004. Epub 2018 Aug 11.
The term Fetal Alcohol Spectrum Disorder (FASD) describes all the deleterious consequences of prenatal alcohol exposure. Impaired social behavior is a common symptom of FASD. The zebrafish has emerged as a powerful model organism with which to examine the effects of embryonic alcohol exposure on social behavior due to an innate strong behavior, called shoaling. The relative transparency of the embryo also makes zebrafish powerful for cellular analyses, such as characterizing neural circuitry. However, as zebrafish develop, pigmentation begins to obscure the brain and other tissues. Due to mutations disrupting pigmentation, the casper zebrafish strain remains relatively transparent throughout adulthood, potentially permitting researchers to image neural circuits in vivo, via epifluorescence, confocal and light sheet microscopy. Currently, however the behavioral profile of casper zebrafish post embryonic alcohol exposure has not been completed. We report that exposure to 1% alcohol from either 6 to 24, or 24 to 26 h postfertilization reduces the social behavior of adult casper zebrafish. Our findings set the stage for the use of this important zebrafish resource in studies of FASD.
胎儿酒精谱系障碍（FASD）一词描述了产前酒精暴露的所有有害后果。社交行为受损是FASD的常见症状。斑马鱼已成为一种强大的模式生物，由于其具有一种称为群聚的天生强烈行为，可用于研究胚胎期酒精暴露对社交行为的影响。胚胎的相对透明性也使斑马鱼在细胞分析方面具有优势，例如对神经回路进行特征描述。然而，随着斑马鱼的发育，色素沉着开始遮盖大脑和其他组织。由于突变破坏了色素沉着，卡斯珀斑马鱼品系在成年后仍相对透明，这可能使研究人员能够通过落射荧光、共聚焦和光片显微镜在体内对神经回路进行成像。然而，目前尚未完成对胚胎期酒精暴露后的卡斯珀斑马鱼行为特征的研究。我们报告称，在受精后6至24小时或24至26小时暴露于1%的酒精中，会降低成年卡斯珀斑马鱼的社交行为。我们的研究结果为在FASD研究中使用这种重要的斑马鱼资源奠定了基础。
