Aikawa T, Matsumoto I, Hirose T, Morikawa T, Tsujimoto Y
Am J Physiol. 1986 May;250(5 Pt 1):E523-9. doi: 10.1152/ajpendo.1986.250.5.E523.
The left adrenal of hypophysectomized-nephrectomized dogs was perfused in situ with artificial medium equilibrated with 95% O2-5% CO2. Repeated secretory responses of aldosterone to ACTH and histamine were almost reproducible during 2 h of perfusion, but those of cortisol and corticosterone gradually reduced. The secretion of aldosterone in response to histamine markedly increased at 1 microM and those of cortisol and corticosterone significantly increased at 1 and 10 microM, respectively. The secretory response of aldosterone to 10 microM histamine was comparable with that to 300 microU/ml ACTH, but the secretory responses of cortisol and corticosterone to 10 microM histamine were very much lower than those to 1 microU/ml ACTH. The secretory responses of these three steroids to 10 microM histamine were almost completely depressed by continuous administration of 10 microM pyrilamine maleate, but not abolished by that of 100 microM metiamide. The secretory responses of these three steroids to 10 microM histamine were slightly depressed by continuous infusion of 10 microM methysergide maleate. These results suggest that histamine at pathophysiological concentrations stimulates the secretion of aldosterone markedly, and those of cortisol and corticosterone slightly, via an H1 receptor of adrenocortical cells in the dog.
对垂体切除-肾切除的犬的左肾上腺进行原位灌注，灌注液为用95% O₂-5% CO₂平衡的人工培养基。在灌注2小时期间，醛固酮对促肾上腺皮质激素（ACTH）和组胺的反复分泌反应几乎可以重现，但皮质醇和皮质酮的分泌反应逐渐降低。组胺浓度为1微摩尔时，醛固酮对组胺的分泌反应显著增加，皮质醇和皮质酮对组胺的分泌反应分别在1微摩尔和10微摩尔时显著增加。醛固酮对10微摩尔组胺的分泌反应与对300微单位/毫升ACTH的反应相当，但皮质醇和皮质酮对10微摩尔组胺的分泌反应远低于对1微单位/毫升ACTH的反应。连续给予10微摩尔马来酸氯苯那敏可几乎完全抑制这三种类固醇对10微摩尔组胺的分泌反应，但连续给予100微摩尔甲硫咪胺则不能消除这种反应。连续输注10微摩尔马来酸美西麦角可使这三种类固醇对10微摩尔组胺的分泌反应略有降低。这些结果表明，在病理生理浓度下，组胺通过犬肾上腺皮质细胞的H1受体显著刺激醛固酮的分泌，并轻微刺激皮质醇和皮质酮的分泌。
