Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Bonn, Germany.
Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh.
Plant Cell Environ. 2018 Dec;41(12):2882-2898. doi: 10.1111/pce.13423. Epub 2018 Sep 17.
Monitoring of ozone damage to crops plays an increasingly important role for the food security of many developing countries. Ethylenediurea (EDU) could be a tool to assess ozone damage to vegetation on field scale, but its physiological mode of action remains unclear. This study investigated mechanisms underlying the ozone-protection effect of EDU in controlled chamber experiments. Ozone sensitive and tolerant rice genotypes were exposed to ozone (108 ppb, 7 hr day ) and control conditions. EDU alleviated ozone effects on plant morphology, foliar symptoms, lipid peroxidation, and photosynthetic parameters in sensitive genotypes. Transcriptome profiling by RNA sequencing revealed that thousands of genes responded to ozone in a sensitive variety, but almost none responded to EDU. Significant interactions between ozone and EDU application occurred mostly in ozone responsive genes, in which up-regulation was mitigated by EDU application. Further experiments documented ozone degrading properties of EDU, as well as EDU deposits on leaf surfaces possibly related to surface protection. EDU application did not mitigate the reaction of plants to other abiotic stresses, including iron toxicity, zinc deficiency, and salinity. This study provided evidence that EDU is a surface protectant that specifically mitigates ozone stress without interfering directly with the plants' stress response systems.
监测臭氧对农作物的损害对许多发展中国家的粮食安全起着越来越重要的作用。乙二醛二脲 (EDU) 可以作为评估田间植被臭氧损害的工具，但它的生理作用机制仍不清楚。本研究在控制室内实验中调查了 EDU 对臭氧保护作用的机制。将对臭氧敏感和耐受的水稻基因型暴露于臭氧 (108 ppb，7 小时/天) 和对照条件下。EDU 减轻了敏感基因型植物形态、叶片症状、脂质过氧化和光合参数对臭氧的影响。通过 RNA 测序进行的转录组分析表明，数千个基因对敏感品种中的臭氧有反应，但几乎没有基因对 EDU 有反应。臭氧和 EDU 应用之间的显著相互作用主要发生在对臭氧有反应的基因中，EDU 应用减轻了这些基因的上调。进一步的实验记录了 EDU 对臭氧的降解特性，以及 EDU 可能与表面保护有关的叶片表面沉积物。EDU 的应用并没有减轻植物对其他非生物胁迫的反应，包括铁毒性、缺锌和盐胁迫。本研究提供的证据表明，EDU 是一种表面保护剂，可特异性减轻臭氧胁迫，而不会直接干扰植物的应激反应系统。
