ATP硫酸化酶的测定
The assay of ATP-sulfurylase.
作者信息
Hommes F A, Moss L
出版信息
Anal Biochem. 1986 Apr;154(1):100-3. doi: 10.1016/0003-2697(86)90501-4.
PMID:3010765
Abstract
An assay method for ATP-sulfurylase is described, based on the incorporation of 35SO4(2-) into adenosine-5'-phosphosulfate (and 3-phosphoadenosine-5'-phosphosulfate) and the separation of these compounds by HPLC. Since the enzyme is easily inactivated at temperatures above 20 degrees C during the assay, the reaction time should not exceed 10 min.
摘要
本文描述了一种ATP硫酸化酶的测定方法，该方法基于将35SO4(2-)掺入腺苷-5'-磷酸硫酸酯（和3-磷酸腺苷-5'-磷酸硫酸酯）并通过高效液相色谱法分离这些化合物。由于该酶在测定过程中于20℃以上的温度下容易失活，因此反应时间不应超过10分钟。
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