McCleary Barry V
Megazyme, Bray, County Wicklow A98 YV29, Ireland.
J AOAC Int. 2018 Aug 14. doi: 10.5740/jaoacint.18-0180.
A method for measurement of total dietary fiber (TDF) has been validated. This method is applicable to plant materials, foods, and food ingredients as consumed, consistent with the 2009 CODEX definition (ALINORM 09/32/REP), and measures insoluble dietary fiber (IDF) and soluble dietary fiber (SDF), comprising SDF that precipitates in the presence of 78% ethanol (SDFP) and SDF that remains soluble in the presence of 78% ethanol (SDFS). The method is an update of AOAC Method 2009.01 and addresses each of the issues identified by analysts in using that method over the past 8 years. A total of 13 laboratories participated in the study, with all laboratories returning valid assay data for most of the 16 test portions (8 blind duplicates) consisting of samples with a range of content of traditional dietary fibers, resistant starch, and nondigestible oligosaccharides. The dietary fiber content of the eight test pairs ranged from 6.90 to 60.37 g/100 g. TDF was calculated as the sum of IDF plus SDFP measured gravimetrically and SDFS measured by HPLC. The repeatability SD ranged from 0.27 to 0.76 g/100 g, and the reproducibility SD ranged from 0.54 to 3.99 g/100 g. The RSD ranged from 1.22 to 6.52%, and the RSD ranged from 2.14 to 10.62%.
一种总膳食纤维（TDF）的测量方法已经得到验证。该方法适用于植物材料、食品以及实际消费的食品成分，符合2009年食品法典定义（ALINORM 09/32/REP），可测量不溶性膳食纤维（IDF）和可溶性膳食纤维（SDF），其中SDF包括在78%乙醇存在下沉淀的SDF（SDFP）以及在78%乙醇存在下仍可溶的SDF（SDFS）。该方法是AOAC方法2009.01的更新版本，解决了分析人员在过去8年使用该方法时发现的每个问题。共有13个实验室参与了这项研究，所有实验室都返回了16个测试部分（8个盲法重复样）中大部分的有效分析数据，这些测试部分由一系列含有传统膳食纤维、抗性淀粉和不可消化寡糖的样品组成。八组测试对的膳食纤维含量范围为6.90至60.37 g/100 g。TDF通过重量法测量的IDF与SDFP之和以及通过HPLC测量的SDFS之和来计算。重复性标准差范围为0.27至0.76 g/100 g，再现性标准差范围为0.54至3.99 g/100 g。相对标准偏差范围为1.22%至6.52%，相对标准偏差范围为2.14%至10.62%。
