Fibercarb, Murrumburrah, Eden Rd, Greystones, County Wicklow A63YW01, Ireland.
Analytical Department, Neogen Ltd, Bray Business Park, Southern Cross Rd, Bray, County Wicklow A98YV29, Ireland.
J AOAC Int. 2022 Dec 22;106(1):127-145. doi: 10.1093/jaoacint/qsac098.
A simple, accurate, and reliable method for the measurement of total dietary fiber (TDF) according to the Codex definition (2009) was developed and successfully validated as AOAC Official Method of Analysis (OMA) 2017.16. Subsequently, OMA 2017.16 was modified to allow separate measurement of soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) fractions.
To perform a collaborative study to evaluate the repeatability and reproducibility of OMA 2017.16 modification for the measurement of total dietary fiber (TDF) as IDF and SDF measured as (1) water SDF that precipitates in 78% aqueous ethanol (SDFP), and (2) water SDF that remains soluble in 78% aqueous ethanol (SDFS) of degree of polymerization ≥3.
Duplicate test portions are incubated with pancreatic α-amylase (PAA), amyloglucosidase (AMG), and protease under the conditions employed in OMA 2017.16. For the measurement of IDF, the digestate is filtered and the IDF determined gravimetrically. SDFP in the IDF filtrate is precipitated with alcohol and captured by filtration and determined. SDFS in the SDFP filtrate is recovered and quantitated by LC. The matrixes included cereal products and flours, vegetables, health food snacks, soup, chocolate, and beans. Additional materials were analyzed by collaborators as "practice samples".
With the diethylene glycol internal standard, all multi-laboratotu (MLV) matrixes resulted in repeatability relative standard deviations (RSDr) for TDF analyses of <3.60% and RSDR ranging from 4.55 to 9.26%. For the practice samples, the RSDR for TDF ranged from 6.69 to 11.68%.
OMA 2022.01 meets the AOAC requirements for repeatability and reproducibility and the data support First Action status.
OMA 2022.01 is a robust and reproducible method for the analysis of insoluble, soluble (SDFP and SDFS), and TDF in a wide range of matrixes.
根据 2009 年食品法典委员会的定义，开发并成功验证了一种简单、准确、可靠的总膳食纤维（TDF）测量方法，该方法已被正式确认为 AOAC 分析方法（OMA）2017.16。随后，OMA 2017.16 进行了修改，允许分别测量可溶膳食纤维（SDF）和不可溶膳食纤维（IDF）的分数。
进行协作研究，以评估 OMA 2017.16 修正案测量总膳食纤维（TDF）为 IDF 和 SDF 的重复性和再现性，SDF 分为（1）在 78%乙醇中沉淀的水可溶膳食纤维（SDFP）和（2）在 78%乙醇中仍可溶的水可溶膳食纤维（SDFS），聚合度≥3。
用胰蛋白酶-α（PAA）、淀粉酶（AMG）和蛋白酶重复处理两份测试样品，酶解条件采用 OMA 2017.16 中所规定的条件。对于 IDF 的测量，将消化物过滤并用重量法测定 IDF。将 IDF 滤液中的 SDFP 用酒精沉淀并用过滤法捕获并测定。将 SDFP 滤液中的 SDFS 回收并通过 LC 定量。基质包括谷物制品和面粉、蔬菜、保健食品小吃、汤、巧克力和豆类。其他材料由合作者作为“实践样品”进行分析。
采用二甘醇内标，所有多实验室（MLV）基质的 TDF 分析重复性相对标准偏差（RSDr）均<3.60%，相对标准偏差（RSDR）范围为 4.55-9.26%。对于实践样品，TDF 的 RSDR 范围为 6.69-11.68%。
OMA 2022.01 满足 AOAC 对重复性和再现性的要求，数据支持首次行动地位。
OMA 2022.01 是一种强大且可重复的方法，可用于分析各种基质中的不溶性、可溶性（SDFP 和 SDFS）和 TDF。
