Department of Systems Biotechnology, Chung-Ang University, Anseong, 17546, Korea.
Korea Polar Research Institute, Incheon, 21990, Korea.
Sci Rep. 2018 Aug 14;8(1):12086. doi: 10.1038/s41598-018-30588-2.
Dandruff is known to be associated with Malassezia restricta. Zinc pyrithione (ZPT) has been used as an ingredient in anti-dandruff treatments. The mechanism of ZPT has been investigated in several studies; however, a non-pathogenic model yeast, such as Saccharomyces cerevisiae was most often used. The aim of the present study was to understand how ZPT inhibits the growth of M. restricta. We analyzed the cellular metal content and transcriptome profile of ZPT-treated M. restricta cells and found that ZPT treatment dramatically increased cellular zinc levels, along with a small increase in cellular copper levels. Moreover, our transcriptome analysis showed that ZPT inhibits Fe-S cluster synthesis in M. restricta. We also observed that ZPT treatment significantly reduced the expression of lipases, whose activities contribute to the survival and virulence of M. restricta on human skin. Therefore, the results of our study suggest that at least three inhibitory mechanisms are associated with the action of ZPT against M. restricta: (i) an increase in cellular zinc levels, (ii) inhibition of mitochondrial function, and (iii) a decrease in lipase expression.
头皮屑与糠秕马拉色菌有关。吡啶硫酮锌（ZPT）已被用作抗头皮屑治疗的成分。ZPT 的作用机制已在多项研究中进行了研究；然而，最常用的是一种非致病性模式酵母，如酿酒酵母。本研究旨在了解 ZPT 如何抑制糠秕马拉色菌的生长。我们分析了 ZPT 处理的糠秕马拉色菌细胞的细胞金属含量和转录组谱，发现 ZPT 处理显着增加了细胞内锌水平，同时细胞内铜水平略有增加。此外，我们的转录组分析表明，ZPT 抑制了糠秕马拉色菌中 Fe-S 簇的合成。我们还观察到，ZPT 处理显着降低了脂酶的表达，其活性有助于糠秕马拉色菌在人体皮肤上的存活和毒力。因此，我们的研究结果表明，ZPT 对糠秕马拉色菌的作用至少涉及三种抑制机制：（i）细胞内锌水平增加，（ii）线粒体功能抑制，和（iii）脂酶表达降低。
