Wang Yiwei, Mo Yanbo, Wang Lin, Su Peng, Xie Yuxi
North China University of Science and Technology Affiliated Hospital, Tangshan 063000, PR China.
North China University of Technology, Tangshan 063000, PR China.
Saudi J Biol Sci. 2018 Jul;25(5):953-958. doi: 10.1016/j.sjbs.2018.03.004. Epub 2018 Mar 6.
Elevated evidences show that microRNAs (miRNAs) play vital roles in tumor progression regulation. However, the functional role of let-7b in hepatocellular carcinoma (HCC) is still largely unknown. In this study, we try to investigate the biological activity of let-7b in human HCC cells and try to find the potential regulatory signaling pathway. Our results indicate that let- 7b was remarkably down-regulated in human HCC tissues by qRT-PCR. In addition, let-7b overexpression decreased the expression of β-catenin and c-Myc, while upregulated E-cadherin expression in HCC cells which was verified by quantitative real-time PCR (qRT-PCR) and western blotting. Furthermore, Wnt/β-catenin was involved in let-7b biological activity which was revealed by luciferase assay. Moreover, Wnt/β-catenin signaling inhibitor blocks HCC cell proliferation which is as the same pattern as let-7b overexpression inhibits in HCC cells proliferation. In conclusion, down-regulated let-7b promotes HCC cell proliferation through Wnt/β-catenin signaling in HCC cells. These results suggested that appropriate manipulation of let-7b might be a new treatment of human HCC in the future.
越来越多的证据表明，微小RNA（miRNA）在肿瘤进展调控中发挥着至关重要的作用。然而，let-7b在肝细胞癌（HCC）中的功能作用仍 largely unknown。在本研究中，我们试图研究let-7b在人肝癌细胞中的生物学活性，并试图找到潜在的调控信号通路。我们的结果表明，通过qRT-PCR检测，let-7b在人肝癌组织中显著下调。此外，let-7b过表达降低了β-catenin和c-Myc的表达，同时上调了肝癌细胞中E-cadherin的表达，这通过定量实时PCR（qRT-PCR）和western blotting得到验证。此外，荧光素酶报告基因检测表明Wnt/β-catenin参与了let-7b的生物学活性。此外，Wnt/β-catenin信号抑制剂阻断肝癌细胞增殖，其模式与let-7b过表达抑制肝癌细胞增殖相同。总之
