T CELL EPITOPES OF THE FULL GENE OF FROM SPUTUM OF MDR-TB PATIENTS.

BACKGROUND

In 2015, World Health Organization (WHO) discovered 10.4 million tuberculosis (TB) cases around the world. Multidrug-resistant tuberculosis (MDR-TB) became a threat because it has high mortality number. There were 480,000 new MDR-TB cases in 2015. Based on those problems, diagnostic development to detect rapidly and accurately is needed. The importance of detecting epitope expression of full gene because there was a potential of complexity over the protein structure and might affect the protein concentration. By knowing epitope prediction, there's an expectation that it can help the development of TB diagnostic. This research was aimed to determine the T cell epitope prediction of full gene from MDR-TB patients.

MATERIAL AND METHODS

Total of 24 MDR-TB sputum isolate from TB patients at Dr. Soetomo Hospital were collected from September to December 2016. Samples were confirmed as MDR-TB using GeneXpert and Bactec MGIT 960. Those samples tested using PCR targeted 580 bp of gene and sequencing. Gene sequence was aligned against wild type using Bioedit program version 7.2.5 and NCBI BLAST. T cell epitope prediction was analyzed by GENETYX version 10.

RESULTS

Epitope predictions that could be obtained were IEAAAS, ASAIQG, VTSIHS, TKLAAA, VTGMFA based IAd Pattern Position and EAAAS based Rothbard/Taylor Pattern Position. Those prediction epitopes can determine the severity of disease, therefore full gene of could be used as diagnostic target.

CONCLUSION

This research discovered five specific T cell epitope prediction based on IAd Pattern Position and one epitope prediction according to Rothbard/Taylor Pattern Position.