缺氧相关 miRNA 210 在淋巴转移前列腺癌原发病灶中的上调。

The upregulation of hypoxia-related miRNA 210 in primary tumor of lymphogenic metastatic prostate cancer.

机构信息

Department of Urology, University Hospital of Cologne, Kerpenerstr. 62, D-50937 Cologne, Germany.

Department of Urology, Stanford University, Stanford, CA 94305, USA.

出版信息

Epigenomics. 2018 Oct;10(10):1347-1359. doi: 10.2217/epi-2017-0114. Epub 2018 Aug 15.

DOI:10.2217/epi-2017-0114

PMID:30109809

Abstract

AIM

To show the association between the expression level of hsa-miR-210 (miR-210) and tumor progression in prostate cancer (PCa).

METHODS

Quantitative PCR was performed to measure miR-210 on 55 subjects with different tumor stages; our results were then validated using three external datasets. ANOVA and Tukey's post hoc analysis were performed for comparative analyses between different tumor stages. Using the transcriptome data from The Cancer Genome Atlas for CaP, the gene expression analyses were performed on experimentally validated target genes of miR-210 identified in Tarbase and miRWalk datasets.

RESULTS & CONCLUSION: miR-210 was significantly higher in N1 PCa compared with nonmetastatic PCa, whereas the metastatic tumor revealed a lower expression level of miR-210 than the primary tumor.

摘要

目的

展示 hsa-miR-210（miR-210）的表达水平与前列腺癌（PCa）肿瘤进展之间的关联。

方法

对 55 名不同肿瘤分期的患者进行定量 PCR 检测 miR-210；然后使用三个外部数据集进行验证。对不同肿瘤分期之间的比较分析进行 ANOVA 和 Tukey 事后分析。使用来自癌症基因组图谱（TCGA）的 CaP 转录组数据，对 Tarbase 和 miRWalk 数据集中确定的 miR-210 的实验验证靶基因进行基因表达分析。