特定酵母序列对大肠杆菌中卡那霉素抗性基因表达的影响

The kanamycin resistance gene expression in Escherichia coli as affected by specific yeast sequences.

作者信息

Polumienko A L, Grigor'eva S P, Lushnikov A A, Domaradskij I V

出版信息

Biochem Biophys Res Commun. 1986 Apr 29;136(2):529-34. doi: 10.1016/0006-291x(86)90472-9.

Abstract

It was shown that the transcription initiation of the kanamycin resistance gene (the Km gene) from transposon Tn5 in E.coli HB101 strain can be provided by specific yeast sequences. To localize a region of the yeast DNA involved in transcription initiation, a number of hybrid plasmids containing promoterless part of the Km gene fused with DNA sequences from chromosome III of the yeast Saccharomyces cerevisiae was constructed. Comparison of nucleotide sequences lying in the LTR of the yeast transposon Tyl-17 with the consensus bacterial promoter revealed strong similarities.

摘要

结果表明，大肠杆菌HB101菌株中转座子Tn5的卡那霉素抗性基因（Km基因）的转录起始可由特定的酵母序列提供。为了定位酵母DNA中参与转录起始的区域，构建了许多杂种质粒，这些质粒含有与酿酒酵母III号染色体DNA序列融合的Km基因无启动子部分。将酵母转座子Tyl-17的LTR中的核苷酸序列与共有细菌启动子进行比较，发现有很强的相似性。