一种分离在大肠杆菌和酵母中具有启动子活性的DNA序列的方法。
A method to isolate DNA sequences that are promoter-active in Escherichia coli and in yeast.
作者信息
Kwak J W, Kim J, Yoo O J, Han M H
机构信息
Genetic Engineering Center, Korea Advanced Institute of Science and Technology, Seoul.
出版信息
Biochem Biophys Res Commun. 1987 Dec 31;149(3):846-51. doi: 10.1016/0006-291x(87)90485-2.
PMID:3322286
Abstract
A method convenient for isolation of DNA sequences capable of directing gene transcription in both organisms of E. coli and yeast is described. The method is composed of sequential steps of phenotypic selection for chloramphenicol resistance, first in E. coli and then in yeast. A series of promoter-probe, shuttle plasmid vectors between yeast and E. coli were constructed and utilized in the method.
摘要
本文描述了一种便于分离能够在大肠杆菌和酵母两种生物体中指导基因转录的DNA序列的方法。该方法包括一系列表型选择步骤，首先在大肠杆菌中选择氯霉素抗性，然后在酵母中选择。构建了一系列酵母和大肠杆菌之间的启动子探针穿梭质粒载体，并用于该方法。
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