Laboratory of Metals in Medicine (MetMed), Department of Chemistry "U. Schiff" , University of Florence , Via della Lastruccia 3 , Postal Code 50019 Sesto Fiorentino , Italy.
Inorg Chem. 2018 Sep 4;57(17):10507-10510. doi: 10.1021/acs.inorgchem.8b02177. Epub 2018 Aug 15.
The reactions of auranofin and three pseudohalide derivatives with bovine serum albumin were explored by ESI-Q-TOF mass spectrometry; a detailed molecular description of the resulting adducts is achieved revealing even subtle differences in reactivity within this series of gold(I) complexes. Our study shows that this kind of investigative approach, formerly applied to the interactions of metal-based drugs with small model proteins of MW 10-15 kDa, e.g., cytochrome c and lysozyme, may now be extended with success to far larger proteins such as serum albumin (MW 66 kDa).
采用 ESI-Q-TOF 质谱法研究了金诺芬和三种拟卤化物衍生物与牛血清白蛋白的反应；通过对生成的加合物进行详细的分子描述，揭示了该系列金（I）配合物中反应性的细微差异。我们的研究表明，这种研究方法以前曾应用于金属基药物与 10-15 kDa 分子量的小模型蛋白（如细胞色素 c 和溶菌酶）的相互作用，现在可以成功扩展到更大的蛋白，如血清白蛋白（分子量 66 kDa）。
