Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, United States of America.
Division of Neurobiology, Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom.
PLoS One. 2018 Aug 15;13(8):e0200759. doi: 10.1371/journal.pone.0200759. eCollection 2018.
The use of genetically encoded 'self-labeling tags' with chemical fluorophore ligands enables rapid labeling of specific cells in neural tissue. To improve the chemical tagging of neurons, we synthesized and evaluated new fluorophore ligands based on Cy, Janelia Fluor, Alexa Fluor, and ATTO dyes and tested these with recently improved Drosophila melanogaster transgenes. We found that tissue clearing and mounting in DPX substantially improves signal quality when combined with specific non-cyanine fluorophores. We compared and combined this labeling technique with standard immunohistochemistry in the Drosophila brain.
利用基因编码的“自标记标签”与化学荧光团配体，可以快速标记神经组织中的特定细胞。为了改善神经元的化学标记，我们合成并评估了基于 Cy、Janelia Fluor、Alexa Fluor 和 ATTO 染料的新荧光团配体，并将其与最近改进的黑腹果蝇转基因进行了测试。我们发现，与特定的非氰基荧光团结合使用时，DPX 组织透明和安装可显著提高信号质量。我们在果蝇大脑中将这种标记技术与标准免疫组织化学进行了比较和组合。
