Munakata H, Isemura M, Yosizawa Z
Biochem Med Metab Biol. 1986 Apr;35(2):179-83. doi: 10.1016/0885-4505(86)90072-1.
The activities of several glycosidases in the lysosomal fraction of the uterine endometrium of rabbit were measured using 4-MU-glycosides as substrates. The specific activity of beta-N-acetylglucosaminidase was the highest, which was followed by beta-galactosidase, beta-glucuronidase, and alpha-galactosidase in this order. beta-Glucosidase had the lowest activity among the glycosidases examined. In order to examine the hormonal effects on these glycosidases, the lysosomal fractions were prepared from the uterine endometrium of the control, estrogen-treated, and progesterone-treated rabbits. In all glycosidases examined, except for beta-glucosidase, the specific activity was highest in the lysosome obtained from estrogen-treated rabbit. The specific activity in the lysosome from the progesterone-treated rabbit was between that from the estrogen-treated rabbit and that from control. Hormonal treatments, however, affected neither pH optimum curves nor isozyme patterns of these glycosidases.
以4-甲基伞形酮糖苷为底物，测定了兔子宫内膜溶酶体组分中几种糖苷酶的活性。β-N-乙酰氨基葡萄糖苷酶的比活性最高，其次依次为β-半乳糖苷酶、β-葡萄糖醛酸酶和α-半乳糖苷酶。在所检测的糖苷酶中，β-葡萄糖苷酶的活性最低。为了研究激素对这些糖苷酶的影响，从对照兔、雌激素处理兔和孕酮处理兔的子宫内膜制备溶酶体组分。在所有检测的糖苷酶中，除β-葡萄糖苷酶外，雌激素处理兔的溶酶体中比活性最高。孕酮处理兔的溶酶体中的比活性介于雌激素处理兔和对照兔之间。然而，激素处理对这些糖苷酶的最适pH曲线和同工酶模式均无影响。
