Hou Wen, Liu Lei, Zhang Xiaohong, Liu Chuanfa
Key Laboratory for Critical Care Medicine of the Ministry of Health, Tianjin First Center Hospital, Tianjin 300192, China.
Organ Transplant Center, Tianjin First Center Hospital, Tianjin 300071, China.
R Soc Open Sci. 2018 Jun 20;5(6):172455. doi: 10.1098/rsos.172455. eCollection 2018 Jun.
Sequential peptide ligation strategy becomes more and more important in large protein or long peptides chemical synthesis due to the limited peptide/protein size obtained by solid phase synthesis of individual peptides or even one-step peptide ligation. Herein, we developed an alternative method which could perform the sequential peptide ligation of several segments from N to C direction based on the combined use of thioacid capture ligation and native chemical ligation. The sweet protein monellin was produced through this strategy on a scale of multi-milligrams.
由于通过单个肽段的固相合成甚至一步肽连接所获得的肽/蛋白质大小有限，因此在大蛋白质或长肽的化学合成中，顺序肽连接策略变得越来越重要。在此，我们开发了一种替代方法，该方法基于硫代酸捕获连接和天然化学连接的联合使用，能够从N端到C端方向进行多个片段的顺序肽连接。通过该策略，以多毫克规模制备了甜味蛋白莫奈林。
