He Li-Juan, Li Jin-Sheng, Chen Xia, Zhang Han-Tao, Zhu Xiao-Guang, Zhang Xiao-di, Xie Su-Hua, Gu Hai-Ou
Beijing Tong Ren Tang Technology Development Co., Ltd.， Pharmaceutical Factory, Beijing 100079, China.
Beijing Zhongyan Tongrentang Medicine R& D Co.， Ltd., National Engineering Research Center for R& D of Traditional Chinese Medicine Multi-ingredient Drugs, Beijing 100079, China.
Zhongguo Zhong Yao Za Zhi. 2018 Jul;43(13):2784-2788. doi: 10.19540/j.cnki.cjcmm.20180408.001.
To study the effect of serum containing Xihuang pill on the proliferation of human breast cancer cell lines MDA-MB-435 and MCF-7 and the gene and protein expressions of Bcl-2, Bax, TP53, in order to explore the effect and mechanism of Xihuang pill in resisting breast cancer. The serum of the rats was prepared by the method of MTT assay. The expressions of Bcl-2 and Bax were detected by RT-PCR. The serum levels of Bcl-2 and Bax and the mRNA expression of TP53 were detected by immunofluorescence. The rats with serum containing Xihuang pill could inhibit the proliferation of MDA-MB-435 cells and MCF-7 cells (<0.05). The serum containing Xihuang pill increased TP53 and Bax in MDA-MB-435 cells (<0.05), and the ratio of Bcl-2/Bax was decreased (<0.05). Meanwhile, the serum containing Xihuang pill could up-regulate the mRNA expression of Bax in MCF-7 cells and decrease the expression of Bcl (<0.05), but there was no significant difference between the expression of TP53mRNA and Bax protein expressions after the treatment of MCF-7 cells with Xihuang pill serum. Serum containing Xihuang pill can induce the apoptosis of human breast cancer cells, and the mechanism of estrogen receptor-negative breast cancer cell apoptosis may be induced by up-regulating the mRNA expression of TP53, which can induce the expression of Bax and promote the metastasis of Bax to mitochondria, and ultimately play the role of inducing apoptosis.
研究含西黄丸血清对人乳腺癌细胞系MDA - MB - 435和MCF - 7增殖及Bcl - 2、Bax、TP53基因和蛋白表达的影响，以探讨西黄丸抗乳腺癌的作用及机制。采用MTT法制备大鼠血清。通过RT - PCR检测Bcl - 2和Bax的表达。通过免疫荧光检测Bcl - 2和Bax的血清水平及TP53的mRNA表达。含西黄丸血清的大鼠可抑制MDA - MB - 435细胞和MCF - 7细胞的增殖（<0.05）。含西黄丸血清可使MDA - MB - 435细胞中TP53和Bax升高（<0.05），Bcl - 2/Bax比值降低（<0.05）。同时，含西黄丸血清可上调MCF - 7细胞中Bax的mRNA表达并降低Bcl的表达（<0.05），但用西黄丸血清处理MCF - 7细胞后，TP53mRNA表达与Bax蛋白表达之间无显著差异。含西黄丸血清可诱导人乳腺癌细胞凋亡，雌激素受体阴性乳腺癌细胞凋亡的机制可能是通过上调TP53的mRNA表达，进而诱导Bax表达并促进Bax向线粒体转移，最终发挥诱导凋亡的作用。
