Li Hong, Zhuang Hai-Lin, Lin Jun-Jin, Zhang Yue-Fen, Huang Hui, Luo Ting, Yu Wen-Tao, Ni Feng
Department of Health Management, Fujian Health College, Fuzhou 350101, China.
Sharing Technology Center, Fujian Medical University, Fuzhou 350122, China.
Zhongguo Zhong Yao Za Zhi. 2018 Aug;43(16):3335-3340. doi: 10.19540/j.cnki.cjcmm.20180508.001.
Rosmarinic acid is one of anti-tumor ingredients in the Sarcandra glabra. After treatment with 0, 10, 30, 90, 270 and 810 μmol·L⁻¹ rosmarinic acid for 24, 48, 72 hours respectively, the inhibitory effects on MCF-7 and MDA-MB-231 cells were observed by CCK-8 and cell wound healing test. No significant inhibition effecton proliferation and migration was observed in MCF-7 cell. However, 90, 270 and 810 μmol·L⁻¹ rosmarinic acid could inhibit the proliferation and migration of MDA-MB-231 cells in a dose-dependent and time-dependent manner. Flow cytometry was further used to detect apoptosis ratios of MDA-MB-231 cells after Annexin V-FITC/PI staining, and significant apoptosis was observed after rosmarinic acid treatment. Real-time PCR and Western blot tests were carried out to detect the expressions of apoptosis-related genes. The down-regulation of the Bcl-2 expression and the up-regulation of the Bax expression were observed in MDA-MB-231 cells after rosmarinic acid treatment. The results suggested that rosmarinicacid can inhibit the proliferation and migration, and induce the apoptosis of MDA-MB-231 cells, which may be correlated with the down-regulation of Bcl-2 gene and the up-regulation of Bax gene.
迷迭香酸是肿节风中的抗肿瘤成分之一。分别用0、10、30、90、270和810 μmol·L⁻¹迷迭香酸处理24、48、72小时后，通过CCK-8和细胞划痕试验观察其对MCF-7和MDA-MB-231细胞的抑制作用。未观察到迷迭香酸对MCF-7细胞的增殖和迁移有显著抑制作用。然而，90、270和810 μmol·L⁻¹迷迭香酸可呈剂量和时间依赖性地抑制MDA-MB-231细胞的增殖和迁移。进一步采用流式细胞术检测Annexin V-FITC/PI染色后MDA-MB-231细胞的凋亡率，发现迷迭香酸处理后细胞出现明显凋亡。进行实时PCR和蛋白质免疫印迹试验以检测凋亡相关基因的表达。迷迭香酸处理后的MDA-MB-231细胞中观察到Bcl-2表达下调和Bax表达上调。结果表明，迷迭香酸可抑制MDA-MB-231细胞的增殖和迁移，并诱导其凋亡，这可能与Bcl-2基因下调和Bax基因上调有关。
