Institute of Occupational Disease Prevention, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing, Jiangsu Province, China.
Nanjing Prevention and Treatment Center for Occupational Disease, Nanjing, Jiangsu Province, China.
Sci Rep. 2018 Aug 15;8(1):12193. doi: 10.1038/s41598-018-29648-4.
This study was conducted to explore the effects of DNMT1 and DNMT3A polymorphisms on susceptibility to noise-induced hearing loss (NIHL) in Chinese workers. A total of 2689 industrial workers from a single textile factory were recruited. Venous blood was collected, as were questionnaire and pure-tone audiometry (PTA) data by specialist physicians. Four selected SNPs (rs7578575, rs749131, rs1550117, and rs2228611) in DNMT1 and DNMT3A were genotyped in 527 NIHL patients and 527 controls. Then, main effects of the genotypes and their interactions were evaluated. Results revealed that the GG genotype at rs749131 and the AG/GG genotypes at rs1550117 and rs2228611 [odds ratio (OR) = 1.87, 2.57, and 1.98 respectively], as well as the haplotypes AGGG and TGGA (rs7578578-rs749131-rs1550117-rs2228611) (OR = 1.35 and 1.56, respectively) were associated with an increased risk of NIHL in the Chinese population. Multifactor dimensionality reduction analysis indicated that rs7578575, rs749131, and rs2228611 interact and are related to increased NIHL risk (OR = 1.63). The genetic polymorphisms rs749131 G, rs1550117 G, and rs2228611 G within the DNMT1 and DNMT3A genes are associated with an increased risk of NIHL in the Chinese population and have the potential to act as biomarkers for noise-exposed workers.
本研究旨在探讨 DNA 甲基转移酶 1（DNMT1）和 3A（DNMT3A）多态性与中国工人噪声性听力损失（NIHL）易感性的关系。研究共纳入了来自某单一纺织厂的 2689 名产业工人。采集静脉血，由专科医生采集问卷和纯音听阈（PTA）数据。在 527 名 NIHL 患者和 527 名对照中，对 DNMT1 和 DNMT3A 中的四个选定单核苷酸多态性（rs7578575、rs749131、rs1550117 和 rs2228611）进行基因分型。然后，评估基因型的主要效应及其相互作用。结果表明，rs749131 的 GG 基因型、rs1550117 和 rs2228611 的 AG/GG 基因型（比值比[OR]分别为 1.87、2.57 和 1.98）以及 rs7578578-rs749131-rs1550117-rs2228611 的 AGGG 和 TGGA 单倍型（OR 分别为 1.35 和 1.56）与中国人群中 NIHL 的风险增加相关。多因子降维分析表明，rs7578575、rs749131 和 rs2228611 相互作用并与增加的 NIHL 风险相关（OR=1.63）。DNMT1 和 DNMT3A 基因内的 rs749131 G、rs1550117 G 和 rs2228611 G 遗传多态性与中国人群中 NIHL 的风险增加相关，并有潜力成为噪声暴露工人的生物标志物。
