Gyeongbuk Institute for Marine Bio-industry, Uljin, Korea.
Division of Biotechnology and Advanced Institute of Environmental and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan-si, Korea.
J Antibiot (Tokyo). 2018 Nov;71(11):920-926. doi: 10.1038/s41429-018-0089-0. Epub 2018 Aug 15.
Yeast biosurfactants have potent applications in medical, cosmeceutical, and food industries due to their specific modes of action, low toxicity, and applicability. In this study, biosurfactant-producing yeasts were screened for various industrial applications. Among them, Aureobasidium pullulans strain A11211-4-57 with potent surfactant activity from fleabane flower, Erigeron annus (L.) pers., was selected. From culture supernatant of strain A11211-4-57, five new low-surface-tension chemicals designated as pullusurfactans A-E were identified through consecutive chromatography steps, involving ODS, silica gel, Sephadex LH-20, and ODS Sep-pak cartridge columns. Based on mass and NMR measurements, structures of pullusurfactans A-E were determined as myo-inositol lipids with molecular formulae of CHO CHO, CHO, CHO, and CHO, respectively. These compounds exhibited potent biosurfactant activities (22.90, 22.40, 32.28, 25.28, and 22.44 mN/m, respectively). These results suggest that these novel biosurfactants have potential use as biosurfactants in industrial aspect.
酵母生物表面活性剂由于其特定的作用模式、低毒性和适用性，在医学、化妆品和食品工业中有广泛的应用。在这项研究中，筛选了具有各种工业应用潜力的产生物表面活性剂酵母。其中，从蒲公英花（Erigeron annus（L.）pers.）中筛选出具有强表面活性剂活性的出芽短梗霉（Aureobasidium pullulans）菌株 A11211-4-57。从菌株 A11211-4-57 的培养上清液中，通过连续的色谱步骤，包括 ODS、硅胶、Sephadex LH-20 和 ODS Sep-pak 柱，鉴定出五种新的低表面张力化合物，命名为 pullusurfactans A-E。根据质谱和 NMR 测量，确定了 pullusurfactans A-E 的结构为肌醇脂质，其分子式分别为 CHOCHO、CHO、CHO、CHO 和 CHO。这些化合物表现出很强的生物表面活性剂活性（分别为 22.90、22.40、32.28、25.28 和 22.44 mN/m）。这些结果表明，这些新型生物表面活性剂在工业方面有作为生物表面活性剂的应用潜力。
