Burke D S, Chatiyanonda K, Anandrik S, Nakornsri S, Nisalak A, Hoke C H
Bull World Health Organ. 1985;63(6):1037-42.
An IgM antibody-capture type enzyme-linked immunoassay (MAC ELISA) was compared with the haemagglutination inhibition method (HI) for establishing a laboratory diagnosis of acute Japanese encephalitis (JE) virus infection using specimens of dried blood eluted from filter paper strips. Paired samples from 243 encephalitis patients, which had been obtained by mail through a national surveillance programme in Thailand, were tested. During the peak of the 1983 encephalitis epidemic, 72% of cases were diagnosed as Japanese encephalitis by MAC ELISA, compared with only 38% by HI. During non-epidemic periods, the proportions diagnosed as Japanese encephalitis by MAC ELISA or HI were 26% and 33%, respectively. Detection of IgM anti-JE activity by the antibody-capture immunoassay is superior to the HI method for establishing a diagnosis of acute Japanese encephalitis using dried blood specimens.
采用从滤纸条洗脱的干血标本,比较了IgM抗体捕获型酶联免疫吸附测定法(MAC ELISA)和血凝抑制法(HI)用于实验室诊断急性日本脑炎(JE)病毒感染的效果。对通过泰国全国监测计划邮寄获得的243例脑炎患者的配对样本进行了检测。在1983年脑炎流行高峰期,MAC ELISA诊断为日本脑炎的病例占72%,而HI仅为38%。在非流行期,MAC ELISA和HI诊断为日本脑炎的比例分别为26%和33%。抗体捕获免疫测定法检测IgM抗JE活性在使用干血标本诊断急性日本脑炎方面优于HI法。