环核苷酸、盐酸倍他唑和乙酰胆碱对离体兔胃黏膜胃蛋白酶原分泌的影响。

Effects of cyclic nucleotides, betazole hydrochloride and acetylcholine on pepsinogen secretion from isolated rabbit gastric mucosa.

作者信息

Noguchi Y, Ito M, Katsumi K, Yokoyama Y, Yasue N, Miyamoto T, Joh T, Takeuchi T

出版信息

Gastroenterol Jpn. 1986 Apr;21(2):129-34. doi: 10.1007/BF02774830.

Abstract

The effects of dibutyryl cyclic AMP (db-cAMP), dibutyryl cyclic GMP (db-cGMP), betazole hydrochloride (betazole) and acetylcholine (ach.) on pepsinogen secretion from isolated rabbit gastric mucosa were studied using an organ culture system. The 10(-3) M db-cAMP, but not db-cGMP of any concentration, produced a significant enhancement of pepsinogen secretion into the culture medium. In the presence of aminophylline (phosphodiesterase inhibitor), betazole stimulated pepsinogen secretion at concentrations of 10(-8), 10(-6) and 10(-4) M. The 10(-4) M betazole stimulated pepsinogen secretion most strongly (208% of control) and 10(-6) M betazole induced submaximal secretion (137% of control). Ach. stimulated pepsinogen secretion at 10(-8), 10(-6), 10(-4) and 10(-2) M. The peak secretion occurred at 10(-4) M ach. (303% of control). Betazole (with aminophylline) against a background of 10(-6) M ach. (submaximal stimulation dose), produced an intense stimulation of pepsinogen secretion at the concentrations of 10(-8), 10(-6) and 10(-4) M, and the secretion rate expressed as percent of control were 277, 350 and 329%, respectively. These responses were not considered the additive action by betazole and ach. but the potential interaction between the two agents in pepsinogen secretion. From these findings, we conclude that betazole-ach. interdependency exists in in vitro pepsinogen secretion.

摘要

利用器官培养系统研究了二丁酰环磷腺苷（db-cAMP）、二丁酰环磷鸟苷（db-cGMP）、盐酸倍他唑（betazole）和乙酰胆碱（ach.）对离体兔胃黏膜胃蛋白酶原分泌的影响。10⁻³M的db-cAMP能显著增强培养基中胃蛋白酶原的分泌，而任何浓度的db-cGMP都无此作用。在氨茶碱（磷酸二酯酶抑制剂）存在的情况下，10⁻⁸、10⁻⁶和10⁻⁴M的倍他唑能刺激胃蛋白酶原分泌。10⁻⁴M的倍他唑刺激胃蛋白酶原分泌的作用最强（为对照的208%），10⁻⁶M的倍他唑引起次最大分泌（为对照的137%）。10⁻⁸、10⁻⁶、10⁻⁴和10⁻²M的乙酰胆碱能刺激胃蛋白酶原分泌。在10⁻⁴M乙酰胆碱时分泌达到峰值（为对照的303%）。在10⁻⁶M乙酰胆碱（次最大刺激剂量）的背景下，10⁻⁸、10⁻⁶和10⁻⁴M的倍他唑能强烈刺激胃蛋白酶原分泌，以对照百分比表示的分泌率分别为277%、350%和329%。这些反应并非倍他唑和乙酰胆碱的相加作用，而是这两种药物在胃蛋白酶原分泌中存在潜在的相互作用。从这些发现中，我们得出结论：在体外胃蛋白酶原分泌中存在倍他唑-乙酰胆碱相互依赖性。