盐酸倍他唑和环磷酸腺苷对器官培养的兔胃黏膜胃蛋白酶原分泌的影响。

Effects of betazole hydrochloride and cyclic AMP on the pepsinogen secretion by rabbit gastric mucosa in organ culture.

作者信息

Ito M, Noguchi Y, Yokochi K, Kishimoto T, Tomomatsu T, Katsumi K, Takeuchi T

出版信息

Digestion. 1982;23(3):169-73. doi: 10.1159/000198724.

DOI:10.1159/000198724

Abstract

The effects of betazole hydrochloride, dibutyryl cyclic AMP (DB-cyclic AMP) and betazole hydrochloride plus aminophylline on pepsinogen secretion by rabbit gastric mucosa were studied in organ culture. Betazole hydrochloride alone did not stimulate pepsinogen secretion at the concentrations of 10(-8), 10(-6), 10(-5) and 10(-4) M. However, 10(-3) M DB-cyclic AMP produced a significant stimulation of pepsinogen secretion into the culture medium when compared with the control. In the presence of 3 x 10(-3) M aminophylline, betazole hydrochloride in the concentration of 10(-5) and 10(-4) M stimulated pepsinogen secretion into the culture medium, and the magnitude of this increase was 2.0- and 2.8-fold, respectively, compared with the control. Aminophylline alone could not change pepsinogen secretion into the culture medium. These results suggested that the pepsinogen secretion, stimulated by betazole hydrochloride, was mediated by cyclic AMP in the chief cells.

摘要

在器官培养中研究了盐酸倍他唑、二丁酰环磷腺苷（DB - 环磷腺苷）以及盐酸倍他唑加氨茶碱对兔胃黏膜胃蛋白酶原分泌的影响。单独使用盐酸倍他唑在10⁻⁸、10⁻⁶、10⁻⁵和10⁻⁴ M浓度时不刺激胃蛋白酶原分泌。然而，与对照组相比，10⁻³ M DB - 环磷腺苷可显著刺激胃蛋白酶原分泌到培养基中。在存在3×10⁻³ M氨茶碱的情况下，10⁻⁵和10⁻⁴ M浓度的盐酸倍他唑刺激胃蛋白酶原分泌到培养基中，与对照组相比，这种增加的幅度分别为2.0倍和2.8倍。单独的氨茶碱不能改变胃蛋白酶原分泌到培养基中的情况。这些结果表明，盐酸倍他唑刺激的胃蛋白酶原分泌是由主细胞中的环磷腺苷介导的。