Emi M, Nakamura Y, Ogawa M, Yamamoto T, Nishide T, Mori T, Matsubara K
Gene. 1986;41(2-3):305-10. doi: 10.1016/0378-1119(86)90111-3.
Two cDNA clones encoding two major human trypsinogen isozymes were isolated from a human pancreatic cDNA library. The deduced amino acid (aa) sequences of the two trypsinogen precursors are found to have 89% sequence homology, and have the same number of aa (247), including 15 aa for a signal peptide and 8 aa for an activation peptide. Southern blot analysis of human genomic DNA using the cloned cDNA as a probe, revealed that the human trypsinogen genes constitute a multigene family of more than ten genes.
从人胰腺cDNA文库中分离出两个编码两种主要人胰蛋白酶原同工酶的cDNA克隆。发现这两种胰蛋白酶原前体的推导氨基酸（aa）序列具有89%的序列同源性，且氨基酸数量相同（247个），包括15个氨基酸的信号肽和8个氨基酸的激活肽。用人基因组DNA进行Southern印迹分析，以克隆的cDNA为探针，结果表明人胰蛋白酶原基因构成一个由十多个基因组成的多基因家族。
