Schmidt B J, Strasser J, Saunders C W
Gene. 1986;41(2-3):331-5. doi: 10.1016/0378-1119(86)90116-2.
A Bacillus subtilis/Escherichia coli shuttle vector was modified to contain the origin of DNA replication of the E. coli filamentous phage f1, in both orientations. Upon superinfection with and f1-related phage of an E. coli strain containing either of the modified vectors, the single-stranded (ss) form of the plasmid was packaged in virions and released to the culture medium. Each of these ss DNAs has been purified from the virions and used as a template for oligodeoxynucleotide-directed mutagenesis. The resulting mutations were demonstrated by DNA sequencing. The capacity of these vectors to be isolated as phage ss DNA from E. coli and to replicate as plasmids in B. subtilis makes them convenient substrates for the production of oligodeoxynucleotide-directed mutations for studies in B. subtilis.
一种枯草芽孢杆菌/大肠杆菌穿梭载体经过改造，使其在两个方向上都含有大肠杆菌丝状噬菌体f1的DNA复制起点。用含有任一改造载体的大肠杆菌菌株被f1相关噬菌体超感染后，质粒的单链（ss）形式被包装在病毒粒子中并释放到培养基中。这些单链DNA中的每一种都已从病毒粒子中纯化出来，并用作寡聚脱氧核苷酸定向诱变的模板。通过DNA测序证实了产生的突变。这些载体能够从大肠杆菌中分离为噬菌体单链DNA，并在枯草芽孢杆菌中作为质粒复制，这使得它们成为用于枯草芽孢杆菌研究的寡聚脱氧核苷酸定向突变产生的便利底物。
