Kaguni J, LaVerne L S, Ray D S
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6250-4. doi: 10.1073/pnas.76.12.6250.
The Escherichia coli DNA replication origin (oriC) and the adjacent asparagine synthetase gene (asnA) have been inserted into the duplex replicative form DNA of the single-stranded phage vector M13Goril. By in vitro recombination, the entire oriC asnA-containing plasmid pJS5 was inserted into M13Gori1 in both possible orientations. Both phage types transduce the asnA gene and confer upon the M13 vector the ability to replicate as a plasmid in the E. coli mutant rep3. In rep+ hosts, these phages undergo single-stranded DNA synthesis and viral morphogenesis.
大肠杆菌DNA复制起点（oriC）及相邻的天冬酰胺合成酶基因（asnA）已被插入单链噬菌体载体M13Goril的双链复制型DNA中。通过体外重组，整个含oriC asnA的质粒pJS5以两种可能的方向插入M13Gori1中。两种噬菌体类型都能转导asnA基因，并赋予M13载体在大肠杆菌突变体rep3中作为质粒进行复制的能力。在rep+宿主中，这些噬菌体进行单链DNA合成和病毒形态发生。
