Mizgirev I V, Safina D R, Demidyuk I V, Kostrov S V
N.N. Petrov National Medical Research Center of Oncology, Ministry of Health of Russia, Leningradskaya Str., 68, Pesochnyy Settlement, St. Petersburg, 197758, Russia.
Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov Sq., 2, Moscow, 123182 , Russia.
Acta Naturae. 2018 Apr-Jun;10(2):24-29.
Development and implementation of adequate organism-level models is one of the key elements in biomedical research that focuses on experimental oncology. Over the last decade, studies using Zebrafish () have gained in popularity in this area of research. This review describes the various approaches that have been used in developing highly effective models for oncological (clinical term, better cancer or tumor) studies based on . Priority is given to transplantation models of cancer and their application to optically transparent lines, including clonal ones, and utilization tumors of various origins bearing fluorescent labels. The combination of tumor transplantation at organism-level models in transparent clonal lines with fluorescent microscopy, FACS-fractionation of tumor cell subsets, and transcription analysis can result in one of the most promising research approaches in providing new information on tumor formation and growth.
开发和实施适当的机体水平模型是专注于实验肿瘤学的生物医学研究的关键要素之一。在过去十年中，使用斑马鱼（Danio rerio）的研究在这一研究领域越来越受欢迎。本综述描述了基于斑马鱼开发高效肿瘤学（临床术语，更好地称为癌症或肿瘤）研究模型所采用的各种方法。重点介绍了癌症移植模型及其在光学透明斑马鱼品系（包括克隆品系）中的应用，以及利用带有荧光标记的各种起源肿瘤。在透明克隆斑马鱼品系的机体水平模型中进行肿瘤移植，结合荧光显微镜、肿瘤细胞亚群的荧光激活细胞分选（FACS）和转录分析，可能会成为提供有关肿瘤形成和生长新信息的最有前景的研究方法之一。
