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重组免疫球蛋白A针对流感病毒血凝素的研发与研究

The Development and Study of Recombinant Immunoglobulin A to Hemagglutinins of the Influenza Virus.

作者信息

Aliev T K, Dement'yeva I G, Toporova V A, Argentova V V, Pozdnyakova L P, Bokov M N, Votchitseva Yu A, Dolgikh D A, Varfolomeyev S D, Sveshnikov P G, Kirpichnikov M P

机构信息

Lomonosov Moscow State University, Department of Chemistry, Leninskie gory 1, bldg. 3, Moscow, 119991, Russia.

Russian Research Center for Molecular Diagnostics and Therapy, Simferopolsky Blvd. 8, Moscow, 117149 , Russia.

出版信息

Acta Naturae. 2018 Apr-Jun;10(2):30-36.

PMID:30116613
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6087826/
Abstract

We obtained recombinant variants of human antibody FI6 broadly specific to hemagglutinins of the influenza A virus. On the basis of a bi-promoter (CMV, hEF1-HTLV) vector, we developed genetic constructs for the expression of the heavy and light chains of the immunoglobulins of IgA1-, IgA2m1-, and IgG-isotypes. Following transfection and selection, stable Chinese hamster ovary (CHO) cell lines were produced. The antibodies of IgA1-, IgA2m1-, and IgG-isotypes were purified from culture media. We performed an immunochemical characterization and studied their interactions with influenza A strains of the H1N1- and H3N2-subtypes. It was shown that recombinant FI6 variants of the IgA-isotype retain the properties of the parental IgG antibody to demonstrate specificity to all the strains tested. The strongest binding was observed for the H1N1 subtype, which belongs to hemagglutinins of phylogenetic group I.

摘要

我们获得了对甲型流感病毒血凝素具有广泛特异性的人源抗体FI6的重组变体。基于双启动子（CMV、hEF1-HTLV）载体，我们构建了用于表达IgA1、IgA2m1和IgG同种型免疫球蛋白重链和轻链的基因构建体。转染和筛选后，产生了稳定的中国仓鼠卵巢（CHO）细胞系。从培养基中纯化了IgA1、IgA2m1和IgG同种型的抗体。我们进行了免疫化学表征，并研究了它们与H1N1和H3N2亚型甲型流感病毒株的相互作用。结果表明，IgA同种型的重组FI6变体保留了亲本IgG抗体的特性，对所有测试菌株均表现出特异性。观察到与属于系统发育I组血凝素的H1N1亚型结合最强。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/752f/6087826/8eeb8447be9c/AN20758251-10-02-030-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/752f/6087826/d91adb73061e/AN20758251-10-02-030-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/752f/6087826/7e3eac60623b/AN20758251-10-02-030-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/752f/6087826/451364dc49e9/AN20758251-10-02-030-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/752f/6087826/16cc76344b24/AN20758251-10-02-030-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/752f/6087826/8eeb8447be9c/AN20758251-10-02-030-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/752f/6087826/d91adb73061e/AN20758251-10-02-030-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/752f/6087826/7e3eac60623b/AN20758251-10-02-030-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/752f/6087826/451364dc49e9/AN20758251-10-02-030-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/752f/6087826/16cc76344b24/AN20758251-10-02-030-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/752f/6087826/8eeb8447be9c/AN20758251-10-02-030-g005.jpg

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