Kitaoku Yoshihito, Courtade Gaston, Petrović Dejan M, Fukamizo Tamo, Eijsink Vincent G H, Aachmann Finn L
Biochemistry-Electrochemistry Research Unit, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.
NOBIPOL, Department of Biotechnology and Food Science, NTNU Norwegian University of Science and Technology, Sem Sælands vei 6/8, 7491, Trondheim, Norway.
Biomol NMR Assign. 2018 Oct;12(2):357-361. doi: 10.1007/s12104-018-9839-y. Epub 2018 Aug 16.
The apo-form of the 24.4 kDa AA9 family lytic polysaccharide monooxygenase TaLPMO9A from Thermoascus aurantiacus has been isotopically labeled and recombinantly expressed in Pichia pastoris. In this paper, we report the H, C, and N chemical shift assignments, as well as an analysis of the secondary structure of the protein based on the secondary chemical shifts.
来自嗜热栖热菌的24.4 kDa AA9家族裂解多糖单加氧酶TaLPMO9A的脱辅基形式已进行同位素标记，并在毕赤酵母中重组表达。在本文中，我们报告了H、C和N化学位移归属，以及基于二级化学位移的蛋白质二级结构分析。
