Endocrine Physiology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Life Sci. 2018 Oct 1;210:76-85. doi: 10.1016/j.lfs.2018.08.032. Epub 2018 Aug 15.
Nitric oxide (NO) deficiency is associated with obesity. Nitrate could act as a substrate for production of NO and is a novel therapeutic agent in obesity. This study aims at determining effects of long-term nitrate administration on obesity indices in normal adult female rats.
Female Wistar rats were divided into four groups (n = 10/each): i.e. control group received tap water and three treatment groups received water containing 50, 100 and 150 mg/L sodium nitrate for 6 months. Body weight (g) was measured monthly; naso-anal length (cm) and obesity indices including body mass index (BMI), Lee index, abdominal and thoracic circumferences were determined every two months. Both white adipose tissue (WAT) and brown adipose tissue (BAT) were weighted and then adiposity index was calculated. In addition, level of NOx (nitrate + nitrite) in serum and adipose tissues were measured at the end of the study.
Compared to controls, body weights and naso-anal length were significantly (P < 0.001) lower in all nitrate-treated rats. Compared to controls, nitrate-treated rats had also lower adiposity indices, BMI, Lee index, abdominal and thoracic circumferences (13%, 17% and 22% for BMI and 5%, 6% and 8% for lee index at dose 50, 100, and 150 mg/L, respectively). In addition, nitrate administration increased NOx levels in serum and adipose tissues.
Long-term nitrate administration has favorable effects on adiposity. It increases brown and decreases white adipose tissues in normal female rats; these observations could potentially help in management of obesity.
一氧化氮（NO）缺乏与肥胖有关。硝酸盐可以作为产生 NO 的底物，是肥胖症的一种新型治疗剂。本研究旨在确定长期给予硝酸盐对正常成年雌性大鼠肥胖指数的影响。
将雌性 Wistar 大鼠分为四组（每组 n=10）：即对照组给予自来水，三组治疗组分别给予含有 50、100 和 150mg/L 硝酸钠的水，持续 6 个月。每月测量体重（g）；每两个月测量鼻肛长（cm）和肥胖指数，包括体重指数（BMI）、李指数、腹围和胸围。同时称重白色脂肪组织（WAT）和棕色脂肪组织（BAT），然后计算肥胖指数。此外，在研究结束时测量血清和脂肪组织中 NOx（硝酸盐+亚硝酸盐）的水平。
与对照组相比，所有接受硝酸盐治疗的大鼠的体重和鼻肛长均显著降低（P<0.001）。与对照组相比，接受硝酸盐治疗的大鼠的肥胖指数、BMI、李指数、腹围和胸围也较低（BMI 分别降低 13%、17%和 22%，Lee 指数分别降低 5%、6%和 8%，剂量为 50、100 和 150mg/L）。此外，硝酸盐给药增加了血清和脂肪组织中的 NOx 水平。
长期给予硝酸盐对肥胖有有利影响。它增加了正常雌性大鼠的棕色脂肪组织，减少了白色脂肪组织；这些观察结果可能有助于肥胖症的管理。
