Hinson J P, Vinson G P, Whitehouse B J, Price G M
J Endocrinol. 1986 May;109(2):279-85. doi: 10.1677/joe.0.1090279.
Using the in-situ, isolated, perfused rat adrenal system, the actions of adrenal stimulants on steroidogenesis and perfusion medium flow rates (under constant perfusion pump conditions) have been studied. In a series of 100 experiments, initial rates of corticosterone output and flow rates were found to be positively correlated, although there was no such relationship between initial rates of aldosterone output and flow rates. Furthermore, in stable perfusion conditions, bolus injections of ACTH increased both flow rate and steroid output in a dose-related manner. In individual experiments there was a clear correlation between corticosterone and flow, but the association between aldosterone secretion rate and flow was less evident. It is possible that this discrepancy arises because of temporal differences in the responses of these two steroids. Flow was also stimulated by dibutyryl cyclic AMP (dbcAMP), with correlations with steroid output similar to ACTH, but the specific zona glomerulosa stimulants angiotensin II amide and potassium ions had, if anything, inhibitory effects on flow, but only at high concentrations. The data suggest that ACTH and dbcAMP evoke specific responses in the adrenal vasculature, resulting in relatively decreased intraglandular vascular resistance. They furthermore suggest that the secretory functions of the inner adrenocortical zones are subject to the additional control of vascular elements in the intact gland.
利用原位、分离、灌注的大鼠肾上腺系统，研究了肾上腺刺激剂对类固醇生成和灌注介质流速（在恒定灌注泵条件下）的作用。在一系列100次实验中，发现皮质酮输出的初始速率与流速呈正相关，尽管醛固酮输出的初始速率与流速之间不存在这种关系。此外，在稳定的灌注条件下，促肾上腺皮质激素的推注以剂量相关的方式增加了流速和类固醇输出。在个别实验中，皮质酮与流速之间存在明显的相关性，但醛固酮分泌速率与流速之间的关联不太明显。这种差异可能是由于这两种类固醇反应的时间差异所致。二丁酰环磷腺苷（dbcAMP）也刺激了流速，与类固醇输出的相关性与促肾上腺皮质激素相似，但特异性的球状带刺激剂血管紧张素II酰胺和钾离子，即便有作用，也只是在高浓度时对流速有抑制作用。数据表明，促肾上腺皮质激素和dbcAMP在肾上腺血管系统中引发特定反应，导致腺体内血管阻力相对降低。数据还表明，完整腺体中肾上腺皮质内区的分泌功能受到血管成分的额外调控。
