Lee Seohyun, Kim Hyuno, Higuchi Hideo
Opt Express. 2018 Jun 25;26(13):16236-16249. doi: 10.1364/OE.26.016236.
The detection of the precise movement of a vesicle during transport in a live cell provides key information for the intracellular delivery process. Here we report a novel numerical method for analyzing three-dimensional vesicle movement. Since the vesicle moves along a linear cytoskeleton during the active transport, our method first detects the orientation and position of the cytoskeleton as a linear section based on angle correlation and linear regression, after noise reduction. Then, the precise vesicle movement is calculated using vector analysis, in terms of rotation angle and translational displacement. Using this method, various vesicle trajectories obtained via high spatiotemporal resolution microscopy can be understood..
在活细胞中运输过程中检测囊泡的精确运动为细胞内递送过程提供了关键信息。在此，我们报告一种用于分析三维囊泡运动的新型数值方法。由于囊泡在主动运输过程中沿着线性细胞骨架移动，我们的方法首先在降噪后基于角度相关性和线性回归将细胞骨架的方向和位置检测为线性部分。然后，使用矢量分析根据旋转角度和平移位移计算囊泡的精确运动。使用这种方法，可以理解通过高时空分辨率显微镜获得的各种囊泡轨迹。
