Institute of Parasitology, Biology Center, Czech Academy of Sciences and Faculty of Sciences, University of South Bohemia, 37005 České Budějovice (Budweis), Czech Republic.
RNA. 2018 Nov;24(11):1594-1606. doi: 10.1261/rna.066233.118. Epub 2018 Aug 17.
MRP1/2 is a heteromeric protein complex that functions in the trypanosomatid mitochondrion as part of the RNA editing machinery, which facilitates multiple targeted insertions and deletions of uridines. MRP1/2 was shown to interact with MRB8170, which initiates RNA editing by marking pre-edited mRNAs, while TbRGG2 is required for its efficient progression on pan-edited mRNAs. Both MRP1/2 and TbRGG2 are capable of modulating RNA-RNA interactions in vitro. As determined by using iCLIP and RIP-qPCR, RNAs bound to MRP1/2 are characterized and compared with those associated with MRB8170 and TbRGG2. We provide evidence that MRP1 and MRB8170 have correlated binding and similar RNA crosslinking peak profiles over minimally and never-edited mRNAs. Our results suggest that MRP1 assists MRB8170 in RNA editing on minimally edited mRNAs.
MRP1/2 是一种异源蛋白复合物，在原生动物线粒体中作为 RNA 编辑机制的一部分发挥作用，该机制促进了多个靶向尿嘧啶的插入和缺失。MRP1/2 被证明与 MRB8170 相互作用，MRB8170 通过标记预编辑的 mRNA 来启动 RNA 编辑，而 TbRGG2 则是其在全编辑 mRNA 上有效进行的必需条件。MRP1/2 和 TbRGG2 都能够在体外调节 RNA-RNA 相互作用。通过使用 iCLIP 和 RIP-qPCR 确定，MRP1/2 结合的 RNA 具有特征，并与与 MRB8170 和 TbRGG2 相关的 RNA 进行了比较。我们提供的证据表明，MRP1 和 MRB8170 在最小编辑和从未编辑的 mRNA 上具有相关的结合和相似的 RNA 交联峰谱。我们的结果表明，MRP1 协助 MRB8170 在最小编辑的 mRNA 上进行 RNA 编辑。
